A Perspective on the Emergence of Sialic Acids as Potent Determinants Affecting Leishmania Biology
Figure 1
Identification of sialic acids on promastigotes of different Leishmania sp. (a) Fluorimetric estimation of enhanced sialic acids on promastigotes of virulent Leishmania sp. as compared to their minimal presence on avirulent UR6. Results are the mean ± S.D. where, * (asterisk) means P < 0.01 as compared to UR6 were performed using as Graph-Pad Prism statistics software (Graph-Pad Software Inc., San Diego, CA, USA) as described in [68, 69]. (b) Differential distribution of 9-O-AcSA on promastigotes as detected fluorimetrically. Results are expressed as 9-O-AcSA (%), defined as the percent of 9-O-AcSA present in the total sialic acid content (μg/5 × 108 promastigotes) as described in [68, 69]. (c) Representative HPLC chromatogram of sialic acids and its derivatives on promastigotes of a few virulent Leishmania sp. and avirulent UR6 of L. donovani (reproduced and adapted from [68, 69] with permission of the publishers, the Cambridge University Press, and Oxford University Press).
