Figure 3: SAG directly stimulates proliferation of T cells. 105 lymphocytes, from normal BALB/c mice (a) and 5 × 104 IL-2-dependent CD8+ cytotoxic T cell line (CTLL-2) were plated in each well and were kept either untreated or treated in vitro with various concentrations of SAG. Proliferation of each type of cells was monitored by 3H thymidine incorporation. Each experiment was performed at least thrice and results are presented as mean ± SD.