Loss of RASSF2 Enhances Tumorigencity of Lung Cancer Cells and Confers Resistance to Chemotherapy
Figure 2
Loss of RASSF2 enhances proliferation and tumorigenicity of lung cancer cells. H441 lung cancer cells were transfected with two independent RASSF2 shRNA constructs and a noneffective shRNA and selected in puromycin for 2 weeks to obtain a population of cells stably expressing the various shRNA constructs. Knockdown of RASSF2 expression was confirmed by Western Blotting (a). Actin was used as a control for protein loading. (b) Growth analysis of the H441 shF2 cells. Cells were harvested and counted at the indicated times to determine cell number. for both shF2-transfected cells compared to control cells. (c) H441 cells stably expressing the shRNA constructs to RASSF2 or control shRNA were plated in soft agar and colony number determined after 14 days. *Statistically different () from cells expressing the control shRNA. The panel on the right shows representative images of the colonies.
