The RASSF1 Gene and the Opposing Effects of the RASSF1A and RASSF1C Isoforms on Cell Proliferation and Apoptosis
Figure 6
Breast T47D and lung NCI-H1299 cancer cells stably overexpressing RASSF1A or RASSF1C were treated with TNF-α and cell lysates were used to assess YAP phosphorylation (pYAP). (a) Breast cancer cells overexpressing RASSF1A showed a small increase in pYAP compared to cells overexpressing RASSF1C but cells overexpressing RASSF1A or RASSF1C showed reduced pYAP compared to control (BB). Overexpression of RASSF1A reduced and RASSF1C increased total YAP in the presence of TNF-α in breast cancer cells. (b) Overexpression of RASSF1A slightly reduced and RASSF1C overexpression slightly increased pYAP protein levels in the presence of TNF-α in lung cancer cells, but pYAP levels in cells overexpressing RASSF1A or RASSF1C are lower compared to those in control cells. Overexpression of RASSF1A slightly reduced and RASSF1C significantly decreased total YAP protein levels in the presence of TNF-α compared to those in control cells. The blots shown represent one of three independent experiments.
