Table of Contents
Molecular Biology International
Volume 2013 (2013), Article ID 189237, 6 pages
http://dx.doi.org/10.1155/2013/189237
Research Article

Investigation of the Association between Genetic Polymorphism of Microsomal Epoxide Hydrolase and Primary Brain Tumor Incidence

1Department of Molecular Biology, Science and Art Faculty, Gaziosmanpasa University, 60150 Tokat, Turkey
2Department of Biochemistry, Faculty of Medicine, Cumhuriyet University, 58100 Sivas, Turkey
3Department of Neurosurgery, Faculty of Medicine, Cumhuriyet University, 58100 Sivas, Turkey

Received 14 August 2013; Revised 11 November 2013; Accepted 12 November 2013

Academic Editor: Mouldy Sioud

Copyright © 2013 Ali Aydin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

mEH is a critical biotransformation enzyme that catalyzes the conversion of xenobiotic epoxide substrates into more polar diol metabolites: it is also capable of inactivating a large number of structurally different molecules. Two polymorphisms affecting enzyme activity have been described in the exon 3 and 4 of the mEH gene. The hypothesis of this study is that inherent genetic susceptibility to a primary brain tumor is associated with mEH gene polymorphisms. The polymorphisms of the mEH gene were determined with PCR-RFLP techniques and 255 Turkish individuals. Our results indicate that the frequency of the mEH exon 4 polymorphism (in controls) is significantly higher than that of primary brain tumor patients (OR = 1.8, 95% CI = 1.0–3.4). This report, however, failed to demonstrate a significant association between mEH exon 3 polymorphism and primary brain tumor susceptibility in this population. Analysis of patients by both histological types of primary brain tumor and gene variants showed no association, although analysis of family history of cancer between cases and controls showed a statistically significant association ( , ). Our results marginally support the hypothesis that genetic susceptibility to brain tumors may be associated with mEPHX gene polymorphisms.