GAPDH Pseudogenes and the Quantification of Feline Genomic DNA Equivalents

Figure 1

Comparison of feline GAPDH and pseudo-GAPDH nucleotide sequences. A comparison of the nucleotide sequences of feline GAPDH mRNA (GenBank: NM_001009307, position 60 to 256, and GenBank: AF097177, position 63 to 228) and gDNA sequences similar to GAPDH retrieved from cat B8 and cat 15. The number listed after the animal identifier (B8 and 15) represents the clone identity. Sequences found in multiple clones are shown only once. Nucleotides that differ from those in the two reference strains are indicated. Primer sequences for the qPCR reported previously [10] were located at positions 29 to 48 and 109 to 93, and the hydrolysis probe spans positions 53 to 89, as indicated. Arrows point to the splicing sites and the intron sequence from the gDNA (Ensembl: ENSFCAG00000006874) is depicted (Intron in gDNA). The exon sequences of the GAPDH gDNA (Ensembl: ENSFCAG00000006874) on which the GAPDH assay is located (exon 2 and 3) are 100% identical to the GAPDH mRNA sequence depicted in this figure (GenBank: NM_001009307).