Error Rate Comparison during Polymerase Chain Reaction by DNA Polymerase
Error rate values for six PCR enzymes included in this study are presented. Each enzyme was used in two independent PCR reactions. Average doublings/PCR reaction () is the average of doubling values for each of the 94 PCR reactions in one plate, where doublings are calculated from the formula = (ng DNA after PCR/ng DNA input). Error rate (f) is calculated as f = / (target size × ), where is the number of mutations observed for all clones that were sequenced and the (target size × for each target that was cloned.