Molecular Biology International / 2014 / Article / Tab 2

Research Article

Error Rate Comparison during Polymerase Chain Reaction by DNA Polymerase

Table 2

Error rate values for six PCR enzymes included in this study are presented. Each enzyme was used in two independent PCR reactions. Average doublings/PCR reaction () is the average of doubling values for each of the 94 PCR reactions in one plate, where doublings are calculated from the formula = (ng DNA after PCR/ng DNA input). Error rate (f) is calculated as f = / (target size ×  ), where is the number of mutations observed for all clones that were sequenced and the (target size ×   for each target that was cloned.

EnzymeExpt.Avg. doublings/PCR reactionNumber of clones sequencedTotal bp sequencedNumber of mutations observedError rate

Taq 120.5 ± 1.2658.8 × 104543.0 × 10−5
216.7 ± 0.7374.7 × 104455.6 × 10−5

AccuPrime-Taq 117.0 ± 1.2751.0 × 105181.0 × 10−5
216.9 ± 0.6N.D.N.D.N.D.N.D.

KOD120.8 ± 1.5701.0 × 105167.6 × 10−6
217.6 ± 0.8N.D.N.D.N.D.N.D.

Pfu (cloned)116.5 ± 1.11512.0 × 10592.8 × 10−6
212.0 ± 1.8N.D.N.D.N.D.N.D.

Phusion121.0 ± 1.91752.4 × 105132.6 × 10−6
216.6 ± 1.1N.D.N.D.N.D.N.D.

Pwo 122.5 ± 1.21702.4 × 105132.4 × 10−6
217.6 ± 0.6N.D.N.D.N.D.N.D.

N.D.: not determined.