Table of Contents
Molecular Biology International
Volume 2014, Article ID 548683, 10 pages
Research Article

The Influence of DNA Extraction Procedure and Primer Set on the Bacterial Community Analysis by Pyrosequencing of Barcoded 16S rRNA Gene Amplicons

Institute of Animal Nutrition, Freie Universität Berlin, Koenigin-Luise-Straße 49, 14195 Berlin, Germany

Received 1 April 2014; Revised 6 June 2014; Accepted 7 June 2014; Published 10 July 2014

Academic Editor: Alessandro Desideri

Copyright © 2014 Ingo C. Starke et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Additional supporting information may be found in the online version of this article:

Table S1: Bar code PCR Primer sequences

Table S2: Percentage of unclassified sequences (n = 12)[%]

Table S3: Mean number of sequences and bacterial genera of unprocessed sequence data detected by three different data bank alignments (n = 12)

  1. Supplementary Material