Table of Contents
Molecular Biology International
Volume 2015 (2015), Article ID 819091, 6 pages
http://dx.doi.org/10.1155/2015/819091
Research Article

Evaluation of Loop-Mediated Isothermal Amplification Assay for the Detection of Pneumocystis jirovecii in Immunocompromised Patients

1Department of Microbiology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, India
2Department of Pulmonary Medicine and Sleep Disorders, AIIMS, Ansari Nagar, New Delhi 110029, India
3Department of Nephrology, AIIMS, Ansari Nagar, New Delhi 110029, India

Received 30 May 2015; Revised 6 September 2015; Accepted 20 October 2015

Academic Editor: Ishita Mukerji

Copyright © 2015 Preeti Singh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Pneumocystis pneumonia (PCP) is one of the common opportunistic infection among HIV and non-HIV immunocompromised patients. The lack of a rapid and specific diagnostic test necessitates a more reliable laboratory diagnostic test for PCP. In the present study, the loop-mediated isothermal amplification (LAMP) assay was evaluated for the detection of Pneumocystis jirovecii. 185 clinical respiratory samples, including both BALF and IS, were subjected to GMS staining, nested PCR, and LAMP assay. Of 185 respiratory samples, 12/185 (6.5%), 41/185 (22.2%), and 49/185 (26.5%) samples were positive by GMS staining, nested PCR, and LAMP assay, respectively. As compared to nested PCR, additional 8 samples were positive by LAMP assay and found to be statistically significant () with the detection limit of 1 pg. Thus, the LAMP assay may serve as a better diagnostic tool for the detection of P. jirovecii with high sensitivity and specificity, less turn-around time, operational simplicity, single-step amplification, and immediate visual detection.