Cloning, Sequencing, and the Expression of the Elusive Sarcomeric TPM4α Isoform in Humans

Figure 6

Western blot analysis of protein extracts from human adult and fetal hearts with CH1 and TPM311 antitropomyosin antibodies. (a) Ponceau-stained blot: lane 1: extract of adult heart; lane 2: extract of fetal heart; lane M: molecular weight markers. (b) Blots stained with CH1 monoclonal antibodies that recognize all sarcomeric TPM isoforms as well as TPM4δ with exon 9a C-terminus end. (c) Blot stained with TM311 monoclonal antibodies that recognize all high molecular wt TPM isoforms with exon 1a at the N-terminus end. However, it does not recognize TPM4δ with exon 1b at the N-terminus end.

(a)

(b)

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