Cloning, Sequencing, and the Expression of the Elusive Sarcomeric TPM4α Isoform in Humans

Figure 8

Identification of amino acid sequences from the peptides extracted from spot G after 2D western blot analyses of adult heart extracts with CH1 antibodies. Red color letters indicate the isolated peptides that have been sequenced by mass spectrometry. (a) TPM1α and (b) TPM4α. Three amino acid residues in exons 1a, 8, and 9a are different in TPM4α marked as bold. In case of TPM1α 76% (red marked) of the total 284 amino acid residues are being identified. For TPM4α it is 38%. It is to be noted that peptide SIDDLEDELYAQKLK in TPM1α was identified by two precursor ions with of 594.07 (3+) and 890.06 (2+) ions. On the other hand, peptide TIDDLEDELYAQKLK in TPM4α was identified by one precursor ion with of 888.06 (2+).

(a)

(b)