Cloning, Sequencing, and the Expression of the Elusive Sarcomeric TPM4α Isoform in Humans

Table 2

Key to 2D gel loading and sample preparation. The sample was lyophilized and redissolved to 4 mg/mL in 1 : 1 diluted SDS boiling buffer : urea sample buffer before loading.

Gel ID #	Sample	μL loaded	μg loaded	Treatment
LF975 #1	HT-801 Zyagen Human Heart	150	500	Coomassie
LF975 #2	HT-801 Zyagen Human Heart	150	500	Antitropomyosin