Soluble Expression and Characterization of Biologically Active Bacillus anthracis Protective Antigen in Escherichia coli

Figure 3

SDS-PAGE analysis of recombinant PA expression from seven different host-vector combinations at different temperature and time interval. Arrow marks indicate recombinant PA expression. (a) PA-pQE30-M15, (b) PA-pQE30-XL-1 blue, (c) PA-pPROEXHTa-DH5α, (d) PA-pPROEXHTa-BL21-DE3, (e) PA-pPROEXHTa-DE3-pLysS, (f) PA-pET32c-BL21-DE3, and (g) PA-pET32c-DE3-pLysS. Lane 1, protein molecular weight marker; lane 2, uninduced fractions; lanes 3, 4, and 5, post-IPTG growth for 3 hours, for 5 hours, and overnight, respectively, at 37°C temperature; lanes 6, 7, and 8, post-IPTG growth for 3 hours, for 5 hours, and overnight, respectively, at 22°C temperature.

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