Soluble Expression and Characterization of Biologically Active Bacillus anthracis Protective Antigen in Escherichia coli

Figure 4

Western blotting analysis of soluble cell lysate from seven different PA expressing clones grown at different temperature and time intervals. (a) PA-pQE30-M15, (b) PA-pQE30-XL-1 blue, (c) PA-pPROEXHTa-DH5α, (d) PA-pPROEXHTa-BL21-DE3, (e) PA-pPROEXHTa-DE3-pLysS, (f) PA-pET32c-BL21-DE3, and (g) PA-pET32c-DE3-pLysS. Lane 1, protein molecular weight marker; lanes 2, 3, and 4, soluble cell lysate from 3 hours, 5 hours, and overnight culture, respectively, at 37°C temperature; lanes 5, 6, and 7, soluble cell lysate from 3 hours, 5 hours, and overnight culture, respectively, at 22°C temperature.

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