Soluble Expression and Characterization of Biologically Active Bacillus anthracis Protective Antigen in Escherichia coli

Table 3

Optimum growth conditions and total yield of recombinant PA protein from different host-vector combinations.

Sl. number	Clones	Optimal growth condition at 1 mmol L^−1 IPTG	Wet pellet weight of one-litre culture	Total yield of soluble protein per litre of culture volume	Total yield of denatured protein per litre of culture volume
1	PA-pQE30-M15	37°C, 5 hours	6.17 g	7.5 mg	6.0 mg
2	PA-pQE30-XL-1 Blue	22°C, overnight	5.49 g	12.0 mg	—
3	PA-pPROEXHTa-DH5	37°C, 5 hours	6.69 g	1.2 mg	11.0 mg
4	PA-pPROEXHTa-BL21 (DE3)	37°C, 5 hours	4.15 g	5.1 mg	28.0 mg
5	PA-pPROEXHTa-DE3-pLysS	22°C, 5 hours	2.94 g	3.0 mg	22.0 mg
6	PA-pET32c-BL21-DE3	22°C, overnight	5.72 g	1.5 mg	36.0 mg
7	PA-pET32c-DE3-pLysS	22°C, 5 hours	6.32 g	15.0 mg	20.0 mg