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Mediators of Inflammation
Volume 1, Issue 5, Pages 309-312

Comparison of the C-mediating killing activity and C-activating properties of mouse monoclonal and polyclonal antibodies against Trypanosoma cruzi

1Departamento de Imunologia, ICB, Universidade de São Paulo Av. Prof. Lineu Prestes 2415, São Paulo CEP 05508-900, Brazil
2Departamento de Bioquimica, IQ, Universidade de São Paulo Av. Prof. Lineu Prestes 748, São Paulo CEP 00508-970, Brazil
3Laboratório de Imunoquimica, Instituto Butantan Av. Vital Brazil 1500, São Paulo CEP 05504-900, Brazil

Copyright © 1992 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A Mouse polyclonal antiserum against Trypanosoma cruzi or its IgG and IgM fractions and five monoclonal antibodies (two IgM, two IgG1 and one IgG2a) recognize and combine with membrane components of trypomastigote forms of the parasite as revealed by immunofluorescence. Although all these antibodies sensitize trypomastigotes and prepare them to activate the complement (C) system, as measured by consumption of total C, C4, B and C3, only the polyclonal antiserum or its IgG, IgM and Fabμ fragments were able to induce trypanosome lysis by the alternative C pathway.