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Mediators of Inflammation
Volume 1 (1992), Issue 5, Pages 329-333

IL-1β and IL-6 modulate apolipoprotein E gene expression in rat hepatocyte primary culture

1CNRS URA 1283, CHU Saint Antoine, 27 rue de Chaligny, Paris 75012, France
2Laboratoire des Protéines de la Réaction Inflammatoire, Faculté de Médecine, 45 rue des Saints Péres, Paris Cedex 06, Paris 75270, France

Copyright © 1992 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Incubation of rat hepatocytes in primary culture with IL-1β at a concentration of 2.5 units/ml resulted in an increase (+80%) in the amount of apoE mRNA without any effect upon apoE synthesis. IL-6 at a low concentration (10 units/ml) induced a decrease (−35%) in the amount of apoE mRNA, but increased apoE synthesis (+28%). No effect was observed with higher concentrations of IL-1β (10 units/ml) or IL-6 (100 units/ml). These results suggest that inflammatory cytokines IL-1β and IL-6 modulate the expression of apoE gene in cultured rat hepatocytes, at a concentration that does not induce the acute phase response.