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Mediators of Inflammation
Volume 3, Issue 2, Pages 161-164

In vitro Superoxide Production by Peripheral Neutrophils from Patients with Inflammatory Bowel Disease

1Department of Medical Gastroenterology C, Herlev Hospital, University of Copenhagen, Herlev Ringvej, Herlev, DK-2730, Denmark
2Department of Infectious Diseases, Ullevål Hospital, University of Oslo, Norway
3Department of Growth and Vascular Biology, Biopharmaceuticals Division, Novo Nordic Ltd, Gentofte, Denmark

Copyright © 1994 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Activated polymorphonuclear leucocytes, which are accumulated in inflammatory lesions of inflammatory bowel disease, produce tissue destructive, oxygen derived free radicals and other inflammatory mediators. The PMN superoxide production elicited by formyl-methionyl-leucyl-phenylalanine or the complement split product 5a were compared in IBD and healthy volunteers. Significantly reduced superoxide production was found in PMNs from patients with Crohn's disease as compared to normal controls, when fMLP or CSa were used as stimulants (p<0.001 and p<0.01, respectively), whereas no differences were found when ulcerative colitis patients were compared to normal controls (p>0.05). The enhanced oxygen derived free radical production previously reported in active IBD, and especially in CD intestinal lesions, may either be due to an accumulation of productive phagocytes or to a change of the inflammatory profile of these cells when migrating into intestinal lesions, possibly due to interaction with other mediators (e.g. adhesion molecules and interleukins).