Changes in Eicosanoid and Tumour Necrosis Factor-α Production
by Rat Peritoneal Macrophages During Carrageenin-Induced Peritonitis
W. M. Pruimboom,1A. Verdoold,1C. J. A. M. Tak,1A. P. M. van Dijk,1M. van Batenburg,1J. H. P. Wilson,2and F. J. Zijlstra1
Abstract
Changes and correlations in cytokine and eicosanoid production by
blood monocytes, non-purified and purified peritoneal cells during a
carrageenin-induced peritonitis were investigated for a period of
ten days. The cells were isolated and stimulated in
vitro. Cytokine and eicosanoid production of the non-purified
fraction increased steadily during peritonitis. During the whole
episode of peritonitis the production capacity of granulocytes was
very low and hardly any effect on the production capacity of
macrophages (Mϕ) was observed. Cytokine and eicosanoid production of the
non-purified fraction was mainly due to the presence of Mϕ. The production capacity of the peripheral blood monocytes was not
similar to that of the peritoneal Mϕ.
