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Mediators of Inflammation
Volume 3 (1994), Issue 1, Pages 53-55
http://dx.doi.org/10.1155/S0962935194000098

Interleukin-5 Potentiates Sulfidopeptide Leukotriene Production by Human Eosinophils

1Department of Pharmacology, Faculty of Pharmacy, Utrecht University, P.O. Box 80.082, Utrecht 3508 TB, The Netherlands
2Central Laboratory of the Netherlands Red Cross, Blood Transfusion Service and Laboratory for Experimental and Clinical Immunology, University of Amsterdam, The Netherlands
3Department of Pulmonary Disease, State University Hospital Utrecht, Utrecht, The Netherlands

Copyright © 1994 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Interleukin-5 (IL-5) has been shown to be a selective eosinophil growth and differentiation factor. In the present study, the effect of recombinant human IL-5 on human eosinophil sulfidopeptide leukotriene production was investigated. IL-5 did not affect leukotriene synthesis in unstimulated eosinophils. However, IL-5 potentiated leukotriene synthesis by eosinophils stimulated with serum treated zymosan (STZ) or the calcium ionophore A23187 by 69% and 135%, respectively. The priming effect of IL-5 was dose dependent, with significant stimulation occurring at 1 000 U/ml for STZ and 100-1 000 U/ml for A23187. Pre-incubation with IL-5 did not increase leukotriene synthesis further.