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Mediators of Inflammation
Volume 3, Issue 4, Pages 291-295
http://dx.doi.org/10.1155/S0962935194000402

Infection with Toxoplasma gondii does not Alter TNFα and IL-6 Secretion by A human Astrocytoma Cell Line

1Dédpartement de Parasitologie-Mycologie Médicale et Moléculaire, CNRS ERS 15, Faculté de Médecine, Université Joseph Fourier, Grenoble 1, Grenoble Cedex, 38043, France
2Laboratoire de Neuro-Biophysique INSERM U 318, Faculté de Médecine, Université Joseph Fourier, Grenoble 1, Grenoble Cedex, 38043, France
3Laboratoire de Biochimie A, Faculté de Médecine, Université Joseph Fourier, Grenoble 1, Grenoble Cedex, 38043, France
4Département de Biophysique CNRS URA 1287, Faculté de Médecine, Université Joseph Fourier, Grenoble 1, Grenoble Cedex, 38043, France

Copyright © 1994 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The secretion of tumour necrosis factor-α (TNFα), interleukin-1α (IL-α) and interleukin-6 (IL-6) by a human astrocytoma cell fine was studied 1 h, 3 h, 6 h and 24 h after infection with tachyzoites from three Toxoplasma gondii strains (virulent, RH; cystogentc, 76K and Prugniaud strains). The astrocytoma cell fine constitutively secreted TNFα and IL-6, but no IL-1α. A positive control was obtained by stimulation with phorbol esters inducing a significant increase (p<0.05) in TNFα and IL- 6 secretion but not in IL-1α, while lipopolysaccharide (alone and after priming), interferon gamma, ionophore A 23187 and sera positive to T. gondii did not induce any increase in cytokine levels. None of the tachyzoites, whatever their virulence, induced a significant increase in cytokine production at any time in the study. Tachyzoites did not inhibit the secretion induced by phorbol esters.