Mediators of Inflammation

Mediators of Inflammation / 1998 / Article

Open Access

Volume 7 |Article ID 192768 |

M. M. Verheggen, P. W. C. Adriaansen-Soeting, C. A. Berrevoets, P. Th. W. van Hal, A. O. Brinkmann, H. C. Hoogsteden, M. A. Versnel, "Glucocorticoid receptor expression in human bronchial epithelial cells: effects of smoking and COPD", Mediators of Inflammation, vol. 7, Article ID 192768, 7 pages, 1998.

Glucocorticoid receptor expression in human bronchial epithelial cells: effects of smoking and COPD


Previously, we found that inflammatory mediators modulated the number and binding affinity of glucocorticoid receptors (GR) in human bronchial epithelial cell lines . In this study we investigated whether smoking and chronic obstructive pulmonary disease (COPD), both characterized by airway inflammation with increased levels of inflammatory mediators , affect GR characteristics in cultured human bronchial epithelial cells (HBEC). A statisticallys ignificant difference was found be tween the dissociation constant (Kd) values in HBEC from smoking (Kd = 0.98 ± 0.08 nM; n=6) and nonsmoking controls (Kd = 0.76 ± 0.10 nM, p=0.03; n=5), but no significant difference was found between the mean number of binding sites . Our results are the first indication that cultured HBEC from smokers possess GR with a lower binding affinity. This may result from the in flammation found in the airways from smokers . Further more, these results provide furthere vidence that the bronchial epithelium may be an actual target for inhaled glucocor ticoid therapy.

Copyright © 1998 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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