M. M. Verheggen, P. W. C. Adriaansen-Soeting, C. A. Berrevoets, P. Th. W. van Hal, A. O. Brinkmann, H. C. Hoogsteden, M. A. Versnel, "Glucocorticoid receptor expression in human bronchial epithelial cells: effects of smoking and COPD", Mediators of Inflammation, vol. 7, Article ID 192768, 7 pages, 1998. https://doi.org/10.1080/09629359890965
Glucocorticoid receptor expression in human bronchial epithelial cells: effects of smoking and COPD
Previously, we found that inflammatory mediators modulated the number and binding affinity of glucocorticoid receptors (GR) in human bronchial epithelial cell lines . In this study we investigated whether smoking and chronic obstructive pulmonary disease (COPD), both characterized by airway inflammation with increased levels of inflammatory mediators , affect GR characteristics in cultured human bronchial epithelial cells (HBEC). A statisticallys ignificant difference was found be tween the dissociation constant (Kd) values in HBEC from smoking (Kd = 0.98 ± 0.08 nM; ) and nonsmoking controls (Kd = 0.76 ± 0.10 nM, ; ), but no significant difference was found between the mean number of binding sites . Our results are the first indication that cultured HBEC from smokers possess GR with a lower binding affinity. This may result from the in flammation found in the airways from smokers . Further more, these results provide furthere vidence that the bronchial epithelium may be an actual target for inhaled glucocor ticoid therapy.
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