Mediators of Inflammation

Mediators of Inflammation / 2001 / Article

Open Access

Volume 10 |Article ID 481504 |

Sue McKay, Mechteld M. Grootte Bromhaar, Johan C. de Jongste, Henk C. Hoogsteden, Pramod R. Saxena, Hari S. Sharma, "Pro-inflammatory cytokines induce c-fos expression followed by IL-6 release in human airway smooth muscle cells", Mediators of Inflammation, vol. 10, Article ID 481504, 8 pages, 2001.

Pro-inflammatory cytokines induce c-fos expression followed by IL-6 release in human airway smooth muscle cells


Background: Airway smooth muscle (ASM) is considered to be a target for mediators released during airway inflammation.Aims: To investigate the expression of c-fos, a constituent of the transcription factor activator protein-1, in human ASM cells. In addition, to measure the release of interleukin (IL)-6 into the conditioned medium of stimulated ASM cells, as well as DNA biosynthesis and changes in cell number.Methods: Serum-deprived human ASM cells in the G0/G1 phase were stimulated with the pro-inflammatory cytokines; tumour necrosis factor-α, IL-1β, IL-5 and IL-6. The expression of mRNA encoding the proto-oncogene c-fos was measured by Northern blot analysis. Cell proliferation was assessed by {3H}-thymidine incorporation assays and cell counting, and IL-6 levels in cell-conditioned medium were measured by enzyme-linked immunosorbent assay.Results: All of the cytokines investigated induced a rapid (within 1h) and transient increase in the expression of mRNA encoding c-fos, followed by the expression and enhanced release of IL-6. Cell proliferation remained unchanged in cytokine-stimulated cells.Conclusions: Cytokine-induced c-fos expression in human ASM cells could be described as a marker of cell ‘activation'. The possible association of these results with airway inflammation, through secondary intracellular mechanisms such as cytokine production, is discussed.

Copyright © 2001 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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