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Mediators of Inflammation
Volume 10 (2001), Issue 5, Pages 245-251

17β-Estradiol promotes the synthesis and the secretion of annexin I in the CCRF-CEM human cell line

1Center for Neuroscience of Coimbra, Department of Zoology, University of Coimbra, Coimbra 3004–517, Portugal
2Faculty of Pharmacy, University of Coimbra, Coimbra, Portugal

Copyright © 2001 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Aims: Annexin I (ANXA1), a 37 kDa member of the annexin family of Ca2+-binding and phospholipidbinding proteins, is particularly abundant in various populations of peripheral blood leukocytes. Since this protein modulates the anti-inflammatory actions of the steroid hormones, the purpose of this study was to investigate the effects of the female sex steroid hormone, 17β-estradiol (E2β), on the synthesis and secretion of ANXA1 in the human CCRF-CEM acute lymphoblastic leukemia cell line.

Methods: Complementary reverse transcription-polymerase chain reaction and Western blot assays were performed to study the effect of E2β on the expression of mRNA and protein ANXA1, respectively.

Results and discussion: Treatment of CCRF-CEM cells with E2β, for 30 min, stimulated the synthesis of ANXA1 mRNA molecules, and increased the cellular level of ANXA1 protein. Moreover, when the cells were incubated with E2β, under the same experimental conditions, a significant increase in the amount of ANXA1 secreted from the cells was also detected. ICI 182,780, a selective inhibitor of the intracellular estrogen receptor, had no effect on the E2β-stimulated expression and externalisation of ANXA1. Taken together, these results indicate that E2β induces de novo synthesis of ANXA1 and stimulates its secretion in the CCRF-CEM cell line, apparently through a mechanism independent of the intracellular estrogen receptor.