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Mediators of Inflammation
Volume 10 (2001), Issue 4, Pages 199-208
http://dx.doi.org/10.1080/09629350123139

Establishment of a consistent L929 bioassay system for TNF-α quantitation to evaluate the effect of lipopolysaccharide, phytomitogens and cytodifferentiation agents on cytotoxicity of TNF-α secreted by adherent human mononuclear cells

1Hung Kuang Institute of Technology, Chung Shan Medical University, Taichung 402, Taiwan
2School of Medical Technology, Chung Shan Medical University, Taichung 402, Taiwan
3Institute of Immunology, Chung Shan Medical University, Taichung 402, Taiwan

Copyright © 2001 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Tumor necrosis factor-α (TNF-α ) plays an important role in the pathogenesis of rheumatoid arthritis. The present study was to evaluate the effects of lipopolysaccharide (LPS), phytomitogens and cytodifferentiation agents on cytotoxicity of TNF-α secreted by adherent human mononuclear cells (AMC). TNF-α cytotoxicity in LPS-treated, phytomitogen-treated, and cytodifferentiation agent-treated AMC supernatants were analyzed by the L929 bioassay system. Our results showed that LPS could induce homogeneous TNF-α production by AMC whereas, in addition to TNF-α, phytomitogens could also induce other TNF-like factors. Neither methotrexate, retinoic acid nor sodium butyrate can inhibit TNF-α cytotoxicity, while hexamethylene bisacetamide could not only inhibit TNF-α cytotoxicity but also TNF-α inducing ability of LPS to AMC.