Mediators of Inflammation

Mediators of Inflammation / 2002 / Article

Open Access

Volume 11 |Article ID 248290 | https://doi.org/10.1080/09629350210000015728

Laura Madrigal-Estebas, Derek G. Doherty, Diarmuid P. O'Donoghue, Conleth Feighery, Cliona O'Farrelly, "Differential expression and upregulation of interleukin-1α, interleukin-1β and interleukin-6 by freshly isolated human small intestinal epithelial cells", Mediators of Inflammation, vol. 11, Article ID 248290, 7 pages, 2002. https://doi.org/10.1080/09629350210000015728

Differential expression and upregulation of interleukin-1α, interleukin-1β and interleukin-6 by freshly isolated human small intestinal epithelial cells

Abstract

Background: Small intestinal epithelial cells (SIEC) may contribute to local immune regulation.Aim: To examine production of interleukin (IL)-1α , IL-1β and IL-6 by freshly isolated human SIEC.Methods: IL-1α and IL-1β mRNA in epithelial layers (EL) prepared from small intestine and in intestinal epithelial cell (EC) lines were examined by reverse transcription-polymerase chain reaction. IL-1α, IL-1β and IL-6 protein expression by SIEC was examined by flow cytometry before and after activation with lipopolysaccharide and epithelial growth factor.Results: IL-1α and IL-1β mRNA was detected in EL and EC lines. Background expression of IL-1α and IL-1β protein by SIEC was observed, which did not increase even following activation. IL-6 protein was expressed by SIEC, in a proportion that increased in two out of three samples following activation.Conclusions: IL-6 expression and the presence of IL-1α and IL-1β mRNA suggest a role for SIEC in the regulation of local inflammation.

Copyright © 2002 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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