Mediators of Inflammation

Mediators of Inflammation / 2002 / Article

Open Access

Volume 11 |Article ID 721589 |

Elena Bacci, Silvana Cianchetti, Stefano Carnevali, Maria Laura Bartoli, Federico L. Dente, Antonella Di Franco, Daniele Giannini, Barbara Vagaggini, Pier Luigi Paggiaro, "Induced sputum is a reproducible method to assess airway inflammation in asthma", Mediators of Inflammation, vol. 11, Article ID 721589, 6 pages, 2002.

Induced sputum is a reproducible method to assess airway inflammation in asthma


To evaluate the reproducibility of induced sputum analysis, and to estimate the sample size required to obtained reliable results, sputum was induced by hypertonic saline inhalation in 29 asthmatic subjects on two different days. The whole sample method was used for analysis, and inflammatory cells were counted on cytospin slides. Reproducibility, expressed by intra-class correlation coefficients, was good for macrophages (+0.80), neutrophils (+0.85), and eosinophils (+0.87), but not for lymphocytes (+0.15). Detectable differences were 5.5% for macrophages, 0.6% for lymphocytes, 5.2% for neutrophils, and 3.0% for eosinophils. We conclude that analysis of induced sputum is a reproducible method to study airway inflammation in asthma. Sample sizes greater than ours give little improvement in the detectable difference of eosinophil percentages.

Copyright © 2002 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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