Mediators of Inflammation

Mediators of Inflammation / 2002 / Article

Open Access

Volume 11 |Article ID 769039 | https://doi.org/10.1080/09629350210000015700

Eduardo Monguilhott Dalmarco, Tânia Silvia Fröde, Yara Santos Medeiros, "Effects of methotrexate upon inflammatory parameters induced by carrageenan in the mouse model of pleurisy", Mediators of Inflammation, vol. 11, Article ID 769039, 8 pages, 2002. https://doi.org/10.1080/09629350210000015700

Effects of methotrexate upon inflammatory parameters induced by carrageenan in the mouse model of pleurisy

Abstract

Background: The model of pleurisy induced by carrageenan exhibits a biphasic response (4 and 48 h) and permits the quantification of exudate, cell migration and certain enzymes such as myeloperoxidase (MPO) and adenosine-deaminase (ADA) that are markers of activated leukocytes.Aims: The present study evaluates whether there exists, in the pleurisy model, a significant inhibition of ADA and MPO enzymes, leukocyte kinetics and other markers of inflammation [nitric oxide (NO) levels, exudation] caused by methotrexate treatment by the intraperitoneal (i.p.) route.Methods: The pleurisy was induced by carrageenan (1%) in mice, and the parameters were analyzed 4 and 48 h after.Results: After the induction of inflammation (4 h), methotrexate (20 mg/kg, i.p., 24 h before pleurisy induction) inhibited the leukocyte infiltration (p<0.05), NO levels and MPO activity (p<0.01), but not ADA activity and fluid leakage (p>0.05). Regarding the second phase of pleurisy (48 h), methotrexate (40 mg/kg, i.p., 0.5 h before pleurisy induction) inhibited the leukocyte infiltration (p<0.05), fluid leakage, NO levels (p<0.01), and ADA and MPO activity (p<0.05).Conclusions: These findings support the evidence that the acute administration of methotrexate has an important systemic anti-inflammatory activity in the studied inflammatory model. This effect was due to a significant inhibition on both neutrophil and mononuclear cells, being less marked in relation to exudation 48 h after. In relation to the enzymes studied and to NO levels, the findings support the evidence that methotrexate inhibits both enzymes (MPO and ADA) from leukocytes at the site of injury, thus reflecting the activation of both neutrophils and lymphocytes, respectively. Furthermore, the inhibiting effect on NO in both phases of pleurisy induced by carrageenan (4 and 48 h) indicates that methotrexate acts on constitutive and/or inducible NO synthases by means of different cells of the pleural cavity.

Copyright © 2002 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


More related articles

 PDF Download Citation Citation
 Order printed copiesOrder
Views208
Downloads759
Citations

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.