Table of Contents Author Guidelines Submit a Manuscript
Mediators of Inflammation
Volume 13, Issue 2, Pages 93-103
http://dx.doi.org/10.1080/09629350410001688486

Involvement of steroids in anti-inflammatory effects of PK11195 in a murine model of pleurisy

1Department of Clinical Analysis, Center of Health Sciences, Universidade Federal de Santa Catarina, Campus Universitário – Trindade, Florianópolis, SC 88040-970, Brazil
2Department of Pharmacology, Center of Biological Sciences, Universidade Federal de Santa Catarina, Campus Universitário – Trindade, Florianópolis, SC 88040-970, Brazil

Copyright © 2004 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

BACKGROUND: Studies on peripheral benzodiazepine receptor function have yielded a diverse list of activities of which the anti-inflammatory effects need to be further examined.

Aims: To evaluate the role of steroids, nitric oxide and adenosine-deaminase in the anti-inflammatory effect of PK11195.

Methods: Pleurisy was induced by intrapleural injection of carrageenan in mice pre-treated or not with PK11195. Leukocytes, exudation, adenosine-deaminase (ADA) activity and nitric oxide (NO) level were measured. Steroid involvement was evaluated by pre-treatment with D,L-aminogluthetimide before PK11195.

Results: Leukocytes, exudation and NO levels were reduced by PK11195 in the early (4 h) phase. In the late (48 h) phase, PK11195 decreased leukocytes and ADA activity. D,L-aminogluthetimide reversed the effect of PK11195 on exudate (4 h), as well as total and differential leukocytes and NO levels (48 h).

Conclusions: Steroids, NO and ADA are implicated in the anti-inflammatory action of PK11195.