Detection of Erythropoietin in Exhaled Breath Condensate of Nonhypoxic Subjects Using a Multiplex Bead Array

Schumann, Christian; Triantafilou, Kathy; Krueger, Stefan; Hombach, Vinzenz; Triantafilou, Martha; Becher, Gunther; Lepper, Philipp M.

doi:https://doi.org/10.1155/MI/2006/18061

Mediators of Inflammation

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Volume 2006 | Article ID 018061 | https://doi.org/10.1155/MI/2006/18061

Detection of Erythropoietin in Exhaled Breath Condensate of Nonhypoxic Subjects Using a Multiplex Bead Array

Christian Schumann,¹Kathy Triantafilou,²Stefan Krueger,¹Vinzenz Hombach,¹Martha Triantafilou,²Gunther Becher,³and Philipp M. Lepper^1,4

Received27 Apr 2006

Revised23 Aug 2006

Accepted23 Aug 2006

Published14 Sept 2006

Abstract

As a noninvasive method, exhaled breath condensate (EBC) has gained importance to improve monitoring of lung diseases and to detect biomarkers. The aim of the study was to investigate, whether erythropoietin (EPO) is detectable in EBC. EBC was collected from 22 consecutive patients as well as from healthy individuals. Using a multiplex fluorescent bead immunoassay, we detected EPO in EBC, as well as tumour necrosis factor-α (TNF-α) in 13 out of 22 patients simultaneously (EPO 0.21 ± 0.03 in U/mL and TNF-α 34.6 ± 4.2 in pg/mL, mean ± SEM). No significant differences for EPO levels or correlation between EPO and TNF-α were found but TNF-α was significantly higher in patients with chronic obstructive pulmonary disease (COPD) than in non-COPD (obstructive sleep apnoea, OSA, and lung healthy patients). This is the first report of detection of EPO in EBC. Due to the small study size more data is needed to clarify the role of EPO in EBC.

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Copyright © 2006 Christian Schumann et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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