Mediators of Inflammation

Mediators of Inflammation / 2006 / Article

Research Communication | Open Access

Volume 2006 |Article ID 028398 | https://doi.org/10.1155/MI/2006/28398

Claudia Heijmans-Antonissen, Feikje Wesseldijk, Renate JM Munnikes, Frank JPM Huygen, Patrick van der Meijden, Wim C. J. Hop, Herbert Hooijkaas, Freek J. Zijlstra, "Multiplex Bead Array Assay for Detection of 25 Soluble Cytokines in Blister Fluid of Patients with Complex Regional Pain Syndrome Type 1", Mediators of Inflammation, vol. 2006, Article ID 028398, 8 pages, 2006. https://doi.org/10.1155/MI/2006/28398

Multiplex Bead Array Assay for Detection of 25 Soluble Cytokines in Blister Fluid of Patients with Complex Regional Pain Syndrome Type 1

Received21 Nov 2005
Accepted31 Dec 2005
Published09 Feb 2006

Abstract

Inflammatory processes are known to be involved at least in the early phase of complex regional pain syndrome type 1 (CRPS1). Blister fluid obtained from the involved extremities displayed increased amounts of proinflammatory cytokines IL-6 and TNFα compared with the noninvolved extremities. The aim of this paper is to investigate the involvement of mediators by measurement of several other cytokines using new detection techniques that enable multiple cytokine measurement in small samples. The use of a multiplex-25 bead array cytokine assay and Luminex technology enabled simultaneous measurement of representative (1) proinflammatory cytokines such as GM-CSF, IL-1β, IL-1RA, IL-6, IL-8, and TNF-α; (2) Th1/Th2 distinguishing cytokines IFN-γ, IL-2, IL-2R, IL-4, IL-5, and IL-10; (3) nonspecific acting cytokines IFN-α, IL-7, IL-12p40/p70, IL-13, IL-15, and IL-17; and (4) chemokines eotaxin, IP-10, MCP-1, MIP-1α, MIP-1β, MIG, and RANTES. Although minimal detection levels are significantly higher in the bead array system than those in common ELISA assays, in blister fluid, IL-1RA, IL-6, IL-8, TNF-α, IL-12p40/p70, MCP-1, and MIP-1β were detectable and increased in CRPS1 affected extremities. Levels of IL-6 and TNF-α simultaneously measured by ELISA (Sanquin Compact kit) and by multiplex-25 bead array assay (Biosource) were highly correlated (r=0.85, P<.001 for IL-6 and r=0.88, P<.001 for TNF-α). Furthermore, IP-10 and eotaxin were detectable but diminished in CRPS1, whereas detectable amounts of IL-10 were similar in involved and noninvolved extremities. Multiplex bead array assays are useful systems to establish the involvement of cytokines in inflammatory processes by measurements in blister fluids of CRPS1. Ten representative cytokines were detectable. However, detection levels and amounts measured are at least 3 times higher in the multiplex-25 array assay than in the ELISA assays used simultaneously for the measurement of cytokines.

References

  1. P Baron, J Schattschneider, A Binder, D Siebrecht, and G Wasner, “Relation between sympathetic vasoconstrictor activity and pain and hyperalgesia in complex regional pain syndromes: a case-control study,” The Lancet, vol. 359, no. 9318, pp. 1655–1660, 2002. View at: Google Scholar
  2. S N Raja and T S Grabow, “Complex regional pain syndrome I (reflex sympathetic dystrophy),” Anesthesiology, vol. 96, no. 5, pp. 1254–1260, 2002. View at: Google Scholar
  3. P HJM Veldman, H M Reynen, I E Arntz, and R JA Goris, “Signs and symptoms of reflex sympathetic dystrophy: prospective study of 829 patients,” The Lancet, vol. 342, no. 8878, pp. 1012–1016, 1993. View at: Google Scholar
  4. W-J T van de Beek, R J Schwartzman, S I van Nes, E M Delhaas, and J J van Hilten, “Diagnostic criteria used in studies of reflex sympathetic dystrophy,” Neurology, vol. 58, no. 4, pp. 522–526, 2002. View at: Google Scholar
  5. S Bruehl, R N Harden, B S Galer et al., “External validation of IASP diagnostic criteria for Complex Regional Pain Syndrome and proposed research diagnostic criteria. International Association for the Study of Pain,” Pain, vol. 81, no. 1-2, pp. 147–154, 1999. View at: Google Scholar
  6. S Bruehl, R N Harden, B S Galer, S Saltz, M Backonja, and M Stanton-Hicks, “Complex regional pain syndrome: are there distinct subtypes and sequential stages of the syndrome?” Pain, vol. 95, no. 1-2, pp. 119–124, 2002. View at: Google Scholar
  7. H M Oerlemans, R A Oostendorp, T de Boo, R S Perez, and R JA Goris, “Signs and symptoms in complex regional pain syndrome type I/reflex sympathetic dystrophy: judgment of the physician versus objective measurement,” The Clinical Journal of Pain, vol. 15, no. 3, pp. 224–232, 1999. View at: Google Scholar
  8. F JPM Huygen, A GJ de Bruijn, J Klein, and F J Zijlstra, “Neuroimmune alterations in the complex regional pain syndrome,” European Journal of Pharmacology, vol. 429, no. 1–3, pp. 101–113, 2001. View at: Google Scholar
  9. F JPM Huygen, A GJ de Bruijn, M T De Bruin, J G Groeneweg, J Klein, and F J Zijistra, “Evidence for local inflammation in complex regional pain syndrome type 1,” Mediators of Inflammation, vol. 11, no. 1, pp. 47–51, 2002. View at: Google Scholar
  10. F JPM Huygen, N Ramdhani, A van Toorenenbergen, J Klein, and F J Zijlstra, “Mast cells are involved in inflammatory reactions during Complex Regional Pain Syndrome type 1,” Immunology Letters, vol. 91, no. 2-3, pp. 147–154, 2004. View at: Google Scholar
  11. F J Zijlstra, I van den Berg-de Lange, F JPM Huygen, and J Klein, “Anti-inflammatory actions of acupuncture,” Mediators of Inflammation, vol. 12, no. 2, pp. 59–69, 2003. View at: Google Scholar
  12. F JPM Huygen, S J Niehof, J Klein, and F J Zijlstra, “Computer-assisted skin videothermography is a highly sensitive quality tool in the diagnosis and monitoring of complex regional pain syndrome type I,” European Journal of Applied Physiology, vol. 91, no. 5-6, pp. 516–524, 2004. View at: Google Scholar
  13. K A O'Connor, A Holguin, M K Hansen, S F Maier, and L R Watkins, “A method for measuring multiple cytokines from small samples,” Brain, Behavior, and Immunity, vol. 18, no. 3, pp. 274–280, 2004. View at: Google Scholar
  14. E B Cook, J L Stahl, L Lowe et al., “Simultaneous measurement of six cytokines in a single sample of human tears using microparticle-based flow cytometry: allergics vs. non-allergics,” Journal of Immunological Methods, vol. 254, no. 1-2, pp. 109–118, 2001. View at: Google Scholar
  15. E Morgan, R Varro, H Sepulveda et al., “Cytometric bead array: a multiplexed assay platform with applications in various areas of biology,” Clinical Immunology, vol. 110, no. 3, pp. 252–266, 2004. View at: Google Scholar
  16. G Hodge, S Hodge, R Haslam et al., “Rapid simultaneous measurement of multiple cytokines using 100 µl sample volumes—association with neonatal sepsis,” Clinical & Experimental Immunology, vol. 137, no. 2, pp. 402–407, 2004. View at: Google Scholar
  17. H Wrigge, U Uhlig, J Zinserling et al., “The effects of different ventilatory settings on pulmonary and systemic inflammatory responses during major surgery,” Anesthesia and Analgesia, vol. 98, no. 3, pp. 775–781, 2004. View at: Google Scholar
  18. M Driessens, H Dijs, G Verheyen, and P Blockx, “What is reflex sympathetic dystrophy?” Acta Orthopaedica Belgica, vol. 65, no. 2, pp. 202–217, 1999. View at: Google Scholar
  19. F JPM Huygen, S J Niehof, F J Zijlstra, P M van Hagen, and P LA van Daele, “Successful treatment of CRPS 1 with anti-TNF,” Journal of Pain and Symptom Management, vol. 27, no. 2, pp. 101–103, 2004. View at: Google Scholar
  20. M Blaha, W Jr Bowers, J Kohl et al., “Effects of CEES on inflammatory mediators, heat shock protein 70A, histology and ultrastructure in two skin models,” Journal of Applied Toxicology, vol. 20, no. suppl 1, pp. S101–S108, 2000. View at: Google Scholar
  21. E Schmidt, B Bastian, R Dummer, H-P Tony, E-B Bröcker, and D Zillikens, “Detection of elevated levels of IL-4, IL-6, and IL-10 in blister fluid of bullous pemphigoid,” Archives for Dermatological Research, vol. 288, no. 7, pp. 353–357, 1996. View at: Google Scholar
  22. O Correia, L Delgado, J-C Roujeau, L Le Cleach, and J A Fleming-Torrinha, “Soluble interleukin 2 receptor and interleukin 1α in toxic epidermal necrolysis: a comparative analysis of serum and blister fluid samples,” Archives of Dermatology, vol. 138, no. 1, pp. 29–32, 2002. View at: Google Scholar
  23. S Ying, Y Kikuchi, Q Meng, A B Kay, and A P Kaplan, “TH1/TH2 cytokines and inflammatory cells in skin biopsy specimens from patients with chronic idiopathic urticaria: comparison with the allergen-induced late-phase cutaneous reaction,” Journal of Allergy and Clinical Immunology, vol. 109, no. 4, pp. 694–700, 2002. View at: Google Scholar
  24. C Günther, G Wozel, J Dreßler, M Meurer, and C Pfeiffer, “Tissue eosinophilia in pemphigoid gestationis: association with eotaxin and upregulated activation markers on transmigrated eosinophils,” American Journal of Reproductive Immunology, vol. 51, no. 1, pp. 32–39, 2004. View at: Google Scholar
  25. M Wakugawa, K Nakamura, H Hino et al., “Elevated levels of eotaxin and interleukin-5 in blister fluid of bullous pemphigoid: correlation with tissue eosinophilia,” British Journal of Dermatology, vol. 143, no. 1, pp. 112–116, 2000. View at: Google Scholar
  26. E Dadfar, J Lundahl, E Fernvik, A Nopp, B Hylander, and S H Jacobson, “Leukocyte CD11b and CD62l expression in response to interstitial inflammation in CAPD patients,” Peritoneal Dialysis International, vol. 24, no. 1, pp. 28–36, 2004. View at: Google Scholar
  27. E Dadfar, J Lundahl, and S H Jacobson, “Monocyte adhesion molecule expression in interstitial inflammation in patients with renal failure,” Nephrology Dialysis Transplantation, vol. 19, no. 3, pp. 614–622, 2004. View at: Google Scholar
  28. L D'Auria, P Cordiali Fei, and F Ameglio, “Cytokines and bullous pemphigoid,” European Cytokine Network, vol. 10, no. 2, pp. 123–134, 1999. View at: Google Scholar
  29. L D'Auria, M Pietravalle, P Cordiali Fei, and F Ameglio, “Increased tryptase and myeloperoxidase levels in blister fluids of patients with bullous pemphigoid: correlations with cytokines, adhesion molecules and anti-basement membrane zone antibodies,” Experimental Dermatology, vol. 9, no. 2, pp. 131–137, 2000. View at: Google Scholar
  30. J V Fahey, T M Schaefer, J Y Channon, and C R Wira, “Secretion of cytokines and chemokines by polarized human epithelial cells from the female reproductive tract,” Human Reproduction, vol. 20, no. 6, pp. 1439–1446, 2005. View at: Google Scholar
  31. S S Khan, M S Smith, D Reda, A F Suffredini, and J P Jr McCoy, “Multiplex bead array assays for detection of soluble cytokines: comparisons of sensitivity and quantitative values among kits from multiple manufacturers,” Cytometry Part B: Clinical Cytometry, vol. 61, no. 1, pp. 35–39, 2004. View at: Google Scholar
  32. W de Jager, H te Velthuis, B J Prakken, W Kuis, and G T Rijkers, “Simultaneous detection of 15 human cytokines in a single sample of stimulated peripheral blood mononuclear cells,” Clinical and Diagnostic Laboratory Immunology, vol. 10, no. 1, pp. 133–139, 2003. View at: Google Scholar

Copyright © 2006 Claudia Heijmans-Antonissen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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