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Mediators of Inflammation
Volume 2006, Article ID 36376, 3 pages
http://dx.doi.org/10.1155/MI/2006/36376
Short Communication

The Quantitative Analysis of bFGF and VEGF by ELISA in Human Meningiomas

1UMR CNRS 6101, 2 rue du Dr. Marcland, Limoges 87025, France
2Service d'Anatomie Pathologique, CHU Dupuytren, 2 Avenue M. Luther King, Limoges 87045, France
3Service de Neurosurgery, CHU Dupuytren, 2 Avenue M. Luther King, Limoges 87045, France

Received 23 June 2006; Revised 3 September 2006; Accepted 10 September 2006

Copyright © 2006 Yves Denizot et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The quantitative analysis of VEGF using ELISA in various subtypes of grade I meningiomas reported higher VEGF contents in meningothelial (2.38±0.62 pg/μg protein, n=7), transitional (1.08±0.21 pg/μg protein, n=13), and microcystic meningiomas (1.98±0.87 pg/μg protein, n=5) as compared with fibrous ones (0.36±0.09 pg/μg protein, n=5). In contrast to VEGF, no difference in the concentrations of bFGF was detected. VEGF levels did not correlate with meningioma grade (1.47±0.23 pg/μg versus 2.29±0.58 pg/μg for 32 and 16 grade I and II, resp), vascularisation (1.53±0.41 pg/μg versus 1.96±0.28 pg/μg for 24 low and 24 high vascularisated tumours, resp), and brain invasion (2.32±0.59 pg/μg versus 1.46±0.27 pg/μg for 7 and 41 patients with and without invasion, resp). The ELISA procedure is, thus, an interesting tool to ensure VEGF and bFGF levels in meningiomas and to test putative correlations with clinical parameters. It is, thus, tempting to speculate that ELISA would also be valuable for the quantitative analysis of other angiogenic growth factors and cytokines in intracranial tumours.