Modification of C<mml:math id="E1" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mi>ɛ</mml:mi></mml:math> mRNA Expression by EBV-Encoded  Latent Membrane Protein 1

Hanashiro, Kazuhiko; Ohta, Shigeto; Sunagawa, Masanori; Nakamura, Mariko; Suzuki, Mikio; Kosugi, Tadayoshi

doi:https://doi.org/10.1155/MI/2006/68069

Mediators of Inflammation

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Research Communication | Open Access

Volume 2006 | Article ID 068069 | https://doi.org/10.1155/MI/2006/68069

Modification of Cɛ mRNA Expression by EBV-Encoded Latent Membrane Protein 1

Kazuhiko Hanashiro,¹Shigeto Ohta,²Masanori Sunagawa,¹Mariko Nakamura,¹Mikio Suzuki,²and Tadayoshi Kosugi¹

Received07 Jun 2006

Revised11 Aug 2006

Accepted14 Aug 2006

Published09 Nov 2006

Abstract

To evaluate the effect of expression of latent membrane protein (LMP) 1 encoded by Epstein-Barr virus (EBV) on Cɛ mRNA expression, mRNA levels were examined by RT-PCR or Northern blot analysis upon transient transfection of LMP1 in the splenocytes derived from Brown-Norway rats with or without immunization with 2,4-dinitrophenyl-conjugated Ascaris suum antigen. Splenocytes were transfected with LMP1 expression vector, pSG5-LMP1, using lipofection method. Cɛ mRNA levels were considerably increased by transfection with pSG5-LMP1 in the splenocytes derived from the nonimmunized rats; however, Cɛ mRNA levels were decreased in the splenocytes derived from the immunized rats. Cɛ mRNA expression in IgE-producing cells are modulated by LMP1, which might depend on the differentiation status of B cells upon exposure to allergen.

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Copyright © 2006 Kazuhiko Hanashiro et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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