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Mediators of Inflammation
Volume 2006, Article ID 79359, 6 pages
http://dx.doi.org/10.1155/MI/2006/79359
Research Communication

Interleukin-1 Modulation of Human Placental Trophoblast Proliferation

Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkla 90112, Thailand

Received 23 November 2005; Accepted 14 December 2005

Copyright © 2006 Athip Nilkaeo and Suthinee Bhuvanath. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

During early pregnancy, interleukin-1 (IL-1) is mainly produced and secreted by maternal decidua. Yet, its biological function on placental cells is not well defined. In this study, we employed JAR choriocarcinoma cell line as a model of human placental trophoblast to study the effect of IL-1. Treatment with recombinant human IL-1β resulted in significant inhibition of JAR proliferation (P<.05) paralleled with increased cytotoxicity. The inhibitory effect was blocked by both IL-1 receptor antagonist (IL-1Ra) and antihuman IL-1β monoclonal antibody. Analyzing the mode of action, IL-1β was found to induce cell cycle arrest in the G0/G1 phase and triggered apoptotic cell death. These findings demonstrated that IL-1 regulates human trophoblast growth by induction of cell cycle delay and cell death.