LIF Upregulates Expression of NK-1R in NHBE Cells

Hu, Cheng-Ping; Feng, Jun-Tao; Tang, Yu-Ling; Zhu, Jin-Qi; Lin, Min-Juan; Yu, Ming-En

doi:https://doi.org/10.1155/MI/2006/84829

Mediators of Inflammation

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Research Communication | Open Access

Volume 2006 | Article ID 084829 | https://doi.org/10.1155/MI/2006/84829

LIF Upregulates Expression of NK-1R in NHBE Cells

Cheng-Ping Hu,¹Jun-Tao Feng,¹Yu-Ling Tang,¹Jin-Qi Zhu,²Min-Juan Lin,¹and Ming-En Yu³

Received24 Mar 2006

Revised30 Jul 2006

Accepted07 Aug 2006

Published13 Sept 2006

Abstract

Leukemia inhibitory factor (LIF), a cytokine at the interface between neurobiology and immunology, is mainly mediated through JAK/STAT pathway and MAPK/ERK pathway. Evidence suggested LIF is related to the higher expression of neurokinin-1 receptor (NK-1R) in asthma. In this study, the immunohistochemistry stain showed the expressions of NK-1R, LIF, p-STAT3, and p-ERK1/2 in the lung tissues of allergic rats were increased compared with the controls, and the main positive cell type was airway epithelial cell. Normal human bronchial epithelial cells were treated with LIF in the presence or absence of AG490 (JAK2 inhibitor), PD98059 (MEK inhibitor), and the siRNA against STAT3. Western blot and RT-PCR indicated that LIF induced the expression of NK-1R, which was inhibited by the inhibitors mentioned above. No significant interaction was found between JAK/STAT pathway and MAPK/ERK pathway. In summary, bronchial epithelial cell changes in asthma are induced by LIF which promotes the expression of NK-1R, and JAK/STAT pathway and MAPK/ERK pathway may participate in this process.

References

C Jo, H Kim, I Jo et al., “Leukemia inhibitory factor blocks early differentiation of skeletal muscle cells by activating ERK,” Biochimica et Biophysica Acta - Molecular Cell Research, vol. 1743, no. 3, pp. 187–197, 2005.
View at: Publisher Site | Google Scholar
W P Schiemann, J L Bartoe, and N M Nathanson, “Box 3-independent signaling mechanisms are involved in leukemia inhibitory factor receptor $α$ - and gp130-mediated stimulation of mitogen-activated protein kinase. Evidence for participation of multiple signaling pathways which converge at Ras,” Journal of Biological Chemistry, vol. 272, no. 26, pp. 16631–16636, 1997.
View at: Publisher Site | Google Scholar
J J Schuringa, S van der Schaaf, and E Vellenga, “LIF-induced STAT3 signaling in murine versus human embryonal carcinoma (EC) cells,” Experimental Cell Research, vol. 274, no. 1, pp. 119–129, 2002.
View at: Publisher Site | Google Scholar
P C Heinrich, I Behrmann, G Muller-Newen, F Schaper, and L Graeve, “Interleukin-6-type cytokine signalling through the gp130/Jak/STAT pathway,” Biochemical Journal, vol. 334, no. 2, pp. 297–314, 1998.
View at: Google Scholar
D P Gearing, M R Comeau, D J Friend et al., “The IL-6 signal transducer, gp130: an oncostatin M receptor and affinity converter for the LIF receptor,” Science, vol. 255, no. 5050, pp. 1434–1437, 1992.
View at: Google Scholar
N Stahl, T G Boulton, T Farruggella et al., “Association and activation of Jak-Tyk kinases by CNTF-LIF-OSM-IL-6 $ß$ receptor components,” Science, vol. 263, no. 5143, pp. 92–95, 1994.
View at: Google Scholar
D A Knight, C P Lydell, D Zhou, T D Weir, R R Schellenberg, and T R Bai, “Leukemia inhibitory factor (LIF) and LIF receptor in human lung distribution and regulation of LIF release,” American Journal of Respiratory Cell and Molecular Biology, vol. 20, no. 4, pp. 834–841, 1999.
View at: Google Scholar
X Zheng, D A Knight, D Zhou et al., “Leukemia inhibitory factor is synthesized and released by human eosinophils and modulates activation state and chemotaxis,” Journal of Allergy and Clinical Immunology, vol. 104, no. 1, pp. 136–144, 1999.
View at: Publisher Site | Google Scholar
P G Jorens, R De Jongh, L L Bossaert et al., “High levels of leukaemia inhibitory factor in ARDS,” Cytokine, vol. 8, no. 11, pp. 873–876, 1996.
View at: Publisher Site | Google Scholar
E J Kim, P J Simpson, D-J Park, B Q Liu, G V Ronnett, and C Moon, “Leukemia inhibitory factor is a proliferative factor for olfactory sensory neurons,” NeuroReport, vol. 16, no. 1, pp. 25–28, 2005.
View at: Publisher Site | Google Scholar
T Yamamori, K Fukada, R Aebersold, S Korsching, M-J Fann, and P H Patterson, “The cholinergic neuronal differentiation factor from heart cells is identical to leukemia inhibitory factor,” Science, vol. 246, no. 4936, pp. 1412–1416, 1989.
View at: Google Scholar
M Murphy, K Reid, M A Brown, and P F Bartlett, “Involvement of leukemia inhibitory factor and nerve growth factor in the development of dorsal root ganglion neurons,” Development, vol. 117, no. 3, pp. 1173–1182, 1993.
View at: Google Scholar
W H Ludlam, K J Chandross, and J A Kessler, “LIF-and IL-1 $β$ -mediated increases in substance P receptor mRNA in axotomized, explanted or dissociated sympathetic ganglia,” Brain Research, vol. 685, no. 1-2, pp. 12–20, 1995.
View at: Publisher Site | Google Scholar
W H Ludlam, Z Zang, K E McCarson, J E Krause, D C Spray, and J A Kessler, “mRNAs encoding muscarinic and substance P receptors in cultured sympathetic neurons are differentially regulated by LIF or CNTF,” Developmental Biology, vol. 164, no. 2, pp. 528–539, 1994.
View at: Publisher Site | Google Scholar
C-P Hu, K Wedde-Beer, A Auais, M M Rodriguez, and G Piedimonte, “Nerve growth factor and nerve growth factor receptors in respiratory syncytial virus-infected lungs,” American Journal of Physiology - Lung Cellular and Molecular Physiology, vol. 283, no. 2, pp. L494–L502, 2002.
View at: Google Scholar
K A King, C-P Hu, M M Rodriguez, R Romaguera, X Jiang, and G Piedimonte, “Exaggerated neurogenic inflammation and substance P receptor upregulation in RSV-infected weanling rats,” American Journal of Respiratory Cell and Molecular Biology, vol. 24, no. 2, pp. 101–107, 2001.
View at: Google Scholar
G Piedimonte, “Contribution of neuroimmune mechanisms to airway inflammation and remodeling during and after respiratory syncytial virus infection,” Pediatric Infectious Disease Journal, vol. 22, no. suppl 2, pp. S66–S75, 2003.
View at: Publisher Site | Google Scholar
D A Knight, A C D'Aprile, L J Spalding, R G Goldie, and P J Thompson, “Leukaemia inhibitory factor (LIF) upregulates excitatory non-adrenergic non-cholinergic and maintains cholinergic neural function in tracheal explants,” British Journal of Pharmacology, vol. 130, no. 5, pp. 975–979, 2000.
View at: Publisher Site | Google Scholar
M-J Lin, X J Lao, H M Ma, and Y-L Tang, “The relationship between leukemia inhibitory factor and neurokinin receptors in a rat model of asthma,” Zhonghua Jie He He Hu Xi Za Zhi, vol. 28, no. 12, pp. 820–824, 2005 (Chinese).
View at: Google Scholar
S O Lee, W Lou, K M Qureshi, F Mehraein-Ghomi, D L Trump, and A C Gao, “RNA interference targeting STAT3 inhibits growth and induces apoptosis of human prostate cancer cells,” The Prostate, vol. 60, no. 4, pp. 303–309, 2004.
View at: Publisher Site | Google Scholar
L Konnikova, M Kotecki, M M Kruger, and B H Cochran, “Knockdown of STAT3 expression by RNAi induces apoptosis in astrocytoma cells,” BMC Cancer, vol. 3, no. 1, pp. 23–31, 2003.
View at: Publisher Site | Google Scholar
P H Patterson, “Leukemia inhibitory factor, a cytokine at the interface between neurobiology and immunology,” Proceedings of the National Academy of Sciences of the United States of America, vol. 91, no. 17, pp. 7833–7835, 1994.
View at: Google Scholar
J A Kessler, M M Freidin, C Kalberg, and K J Chandross, “Cytokines regulate substance P expression in sympathetic neurons,” Regulatory Peptides, vol. 46, no. 1-2, pp. 70–75, 1993.
View at: Publisher Site | Google Scholar
D A Knight, K McKay, B Wiggs, R R Schellenberg, and T R Bai, “Localization of leukaemia inhibitory factor to airway epithelium and its amplification of contractile responses to tachykinins,” British Journal of Pharmacology, vol. 120, no. 5, pp. 883–891, 1997.
View at: Google Scholar
M Schuiling, A B Zuidhof, J Zaagsma, and H Meurs, “Involvement of tachykinin NK1 receptor in the development of allergen-induced airway hyperreactivity and airway inflammation in conscious, unrestrained guinea pigs,” American Journal of Respiratory and Critical Care Medicine, vol. 159, no. 2, pp. 423–430, 1999.
View at: Google Scholar
C Schindler and J E Jr Darnell, “Transcriptional responses to polypeptide ligands: the JAK-STAT pathway,” Annual Review of Biochemistry, vol. 64, pp. 621–651, 1995.
View at: Publisher Site | Google Scholar
T Fukada, M Hibi, Y Yamanaka et al., “Two signals are necessary for cell proliferation induced by a cytokine receptor gp130: involvement of STAT3 in anti-apoptosis,” Immunity, vol. 5, no. 5, pp. 449–460, 1996.
View at: Publisher Site | Google Scholar
K Nishida, Y Yoshida, M Itoh et al., “Gab-family adapter proteins act downstream of cytokine and growth factor receptors and T- and B-cell antigen receptors,” Blood, vol. 93, no. 6, pp. 1809–1816, 1999.
View at: Google Scholar
M Takahashi-Tezuka, Y Yoshida, T Fukada et al., “Gab1 acts as an adapter molecule linking the cytokine receptor gp130 to ERK mitogen-activated protein kinase,” Molecular and Cellular Biology, vol. 18, no. 7, pp. 4109–4117, 1998.
View at: Google Scholar
J-I Park, C J Strock, D W Ball, and B D Nelkin, “The Ras/Raf/MEK/extracellular signal-regulated kinase pathway induces autocrine-paracrine growth inhibition via the leukemia inhibitory factor/JAK/STAT pathway,” Molecular and Cellular Biology, vol. 23, no. 2, pp. 543–554, 2003.
View at: Publisher Site | Google Scholar
J-I Park, C J Strock, D W Ball, and B D Nelkin, “Interleukin-1 $β$ can mediate growth arrest and differentiation via the leukemia inhibitory factor/JAK/STAT pathway in medullary thyroid carcinoma cells,” Cytokine, vol. 29, no. 3, pp. 125–134, 2005.
View at: Publisher Site | Google Scholar
J-I Park, J F Powers, A S Tischler, C J Strock, D W Ball, and B D Nelkin, “GDNF-induced leukemia inhibitory factor can mediate differentiation via the MEK/ERK pathway in pheochromocytoma cells derived from nf1-heterozygous knockout mice,” Experimental Cell Research, vol. 303, no. 1, pp. 79–88, 2005.
View at: Publisher Site | Google Scholar
H Kodama, K Fukuda, J Pan et al., “Significance of ERK cascade compared with JAK/STAT and PI3-K pathway in gp130-mediated cardiac hypertrophy,” American Journal of Physiology - Heart and Circulatory Physiology, vol. 279, no. 4, pp. H1635–H1644, 2000.
View at: Google Scholar
T R Bai, D Zhou, T Weir et al., “Substance P (NK1)- and neurokinin A (NK2)-receptor gene expression in inflammatory airway diseases,” American Journal of Physiology, vol. 95, no. 269, pp. L309–L317, 1995.
View at: Google Scholar
X-F Deng, D G Rokosh, and P C Simpson, “Autonomous and growth factor-induced hypertrophy in cultured neonatal mouse cardiac myocytes: comparison with rat,” Circulation Research, vol. 87, no. 9, pp. 781–788, 2000.
View at: Google Scholar

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Copyright © 2006 Cheng-Ping Hu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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