Curcumin (1,7-bis(4-hydroxy-3-methoxyphenyl)-1, 6-heptadiene-3,5-dione) Blocks the  Chemotaxis of  Neutrophils by Inhibiting Signal Transduction  through IL-8 Receptors

Takahashi, Masafumi; Ishiko, Takatoshi; Kamohara, Hidenobu; Hidaka, Hideaki; Ikeda, Osamu; Ogawa, Michio; Baba, Hideo

doi:https://doi.org/10.1155/2007/10767

Mediators of Inflammation

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Research Article | Open Access

Volume 2007 | Article ID 010767 | https://doi.org/10.1155/2007/10767

Curcumin (1,7-bis(4-hydroxy-3-methoxyphenyl)-1, 6-heptadiene-3,5-dione) Blocks the Chemotaxis of Neutrophils by Inhibiting Signal Transduction through IL-8 Receptors

Masafumi Takahashi,¹Takatoshi Ishiko,¹Hidenobu Kamohara,¹Hideaki Hidaka,²Osamu Ikeda,¹Michio Ogawa,³and Hideo Baba¹

Received22 Oct 2006

Revised24 Jan 2007

Accepted05 Apr 2007

Published26 Jun 2007

Abstract

We investigated the impact of curcumin on neutrophils. Chemotactic activity via human recombinant IL-8 (hrIL-8) was significantly inhibited by curcumin. Curcumin reduced calcium ion flow induced by internalization of the IL-8 receptor. We analyzed flow cytometry to evaluate the status of the IL-8 receptor after curcumin treatment. The change in the distribution of receptors intracellularly and on the cell surface suggested that curcumin may affect the receptor trafficking pathway intracellulary. Rab11 is a low molecular weight G protein associated with the CXCR recycling pathway. Following curcumin treatment, immunoprecipitation studies showed that the IL-8 receptor was associated with larger amounts of active Rab11 than that in control cells. These data suggest that curcumin induces the stacking of the Rab11 vesicle complex with CXCR1 and CXCR2 in the endocytic pathway. The mechanism for antiinflammatory response by curcumin may involve unique regulation of the Rab11 trafficking molecule in recycling of IL-8 receptors.

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Copyright © 2007 Masafumi Takahashi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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