Resting Tension Affects eNOS Activity in a Calcium-Dependent Way in Airways

Kitsiopoulou, Eudoxia; Hatziefthimiou, Apostolia A.; Gourgoulianis, Konstantinos I.; Molyvdas, Paschalis-Adam

doi:https://doi.org/10.1155/2007/24174

Mediators of Inflammation

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Research Article | Open Access

Volume 2007 | Article ID 024174 | https://doi.org/10.1155/2007/24174

Resting Tension Affects eNOS Activity in a Calcium-Dependent Way in Airways

Eudoxia Kitsiopoulou,¹Apostolia A. Hatziefthimiou,¹Konstantinos I. Gourgoulianis,²and Paschalis-Adam Molyvdas¹

Received14 Dec 2006

Accepted05 Feb 2007

Published28 Mar 2007

Abstract

The alteration of resting tension (RT) from 0.5 g to 2.5 g increased significantly airway smooth muscle contractions induced by acetylcholine (ACh) in rabbit trachea. The decrease in extracellular calcium concentration [Ca2+]o from 2 mM to 0.2 mM reduced ACh-induced contractions only at 2.5 g RT with no effect at 0.5 g RT. The nonselective inhibitor of nitric oxide synthase (NOS), NG-nitro-L-arginine methyl ester (L-NAME) increased ACh-induced contractions at 2.5 g RT. The inhibitor of inducible NOS, S-methylsothiourea or neuronal NOS, 7-nitroindazole had no effect. At 2.5 g RT, the reduction of [Ca2+]o from 2 mM to 0.2 mM abolished the effect of L-NAME on ACh-induced contractions. The NO precursor L-arginine or the tyrosine kinase inhibitors erbstatin A and genistein had no effect on ACh-induced contractions obtained at 2.5 g RT. Our results suggest that in airways, RT affects ACh-induced contractions by modulating the activity of epithelial NOS in a calcium-dependent, tyrosine-phosphorylation-independent way.

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Copyright © 2007 Eudoxia Kitsiopoulou et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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