Table of Contents Author Guidelines Submit a Manuscript
Mediators of Inflammation
Volume 2007, Article ID 36381, 6 pages
http://dx.doi.org/10.1155/2007/36381
Research Article

Protective Role of Genistein in Acute Liver Damage Induced by Carbon Tetrachloride

1Department of Internal Medicine, School of Medicine, Firat University, Elazig 23119, Turkey
2Department of Biochemistry, School of Medicine, Firat University, Elazig 23119, Turkey
3Department of Animal Nutrition, School of Veterinary, Firat University, Elazig 23119, Turkey
4Department of Animal Nutrition, School of Medicine, Firat University, Elazig 23119, Turkey
5Divison of Gastroenterology, School of Medicine, Firat University, Elazig 23119, Turkey
6Department of Pathology, School of Medicine, Firat University, Elazig 23119, Turkey

Received 14 October 2006; Revised 18 February 2007; Accepted 19 February 2007

Copyright © 2007 Nalan Kuzu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aim. In the present study, we investigated the protective effect of genistein in experimental acute liver damage induced by CCl4. Method. Forty rats were equally allocated to 5 groups. The first group was designated as the control group (group 1). The second group was injected with intraperitoneal CCl4 for 3 days (group 2). The third group was injected with subcutaneous 1 mg/kg genistein for 4 days starting one day before CCl4 injection. The fourth group was injected with intraperitoneal CCl4 for 7 days. The fifth group was injected with subcutaneous 1 mg/kg genistein for 8 days starting one day before CCl4 injection. Plasma and liver tissue malondialdehyde (MDA) and liver glutathione levels, as well as AST and ALT levels were studied. A histopathological examination was conducted. Results. Liver tissue MDA levels were found significantly lower in group 3, in comparison to group 2 (P<.05). Liver tissue MDA level in group 5 was significantly lower than that in group 4 (P<.001). Liver tissue glutathione levels were higher in group 5 and 3, relative to groups 4 and 2, respectively (P>.05 for each). Inflammation and focal necrosis decreased in group 3, in comparison to group 2 (P<.001 for each). Inflammation and focal necrosis in group 5 was lower than that in group 4 (P<.001). Actin expression decreased significantly in group 5, relative to group 4 (P<.05). Conclusion. Genistein has anti-inflammatory and antinecrotic effects on experimental liver damage caused by CCl4. Genistein reduces liver damage by preventing lipid peroxidation and strengthening antioxidant systems.