Protective Role of Genistein in Acute Liver Damage Induced by Carbon Tetrachloride

Kuzu, Nalan; Metin, Kerem; Dagli, Adile Ferda; Akdemir, Fatih; Orhan, Cemal; Yalniz, Mehmet; Ozercan, Ibrahim Hanifi; Sahin, Kazim; Bahcecioglu, Ibrahim Halil

doi:https://doi.org/10.1155/2007/36381

Mediators of Inflammation

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Research Article | Open Access

Volume 2007 | Article ID 036381 | https://doi.org/10.1155/2007/36381

Protective Role of Genistein in Acute Liver Damage Induced by Carbon Tetrachloride

Nalan Kuzu,¹Kerem Metin,²Adile Ferda Dagli,²Fatih Akdemir,³Cemal Orhan,⁴Mehmet Yalniz,⁵Ibrahim Hanifi Ozercan,⁶Kazim Sahin,⁴and Ibrahim Halil Bahcecioglu⁵

Received14 Oct 2006

Revised18 Feb 2007

Accepted19 Feb 2007

Published05 Apr 2007

Abstract

Aim. In the present study, we investigated the protective effect of genistein in experimental acute liver damage induced by CCl4. Method. Forty rats were equally allocated to 5 groups. The first group was designated as the control group (group 1). The second group was injected with intraperitoneal CCl4 for 3 days (group 2). The third group was injected with subcutaneous 1 mg/kg genistein for 4 days starting one day before CCl4 injection. The fourth group was injected with intraperitoneal CCl4 for 7 days. The fifth group was injected with subcutaneous 1 mg/kg genistein for 8 days starting one day before CCl4 injection. Plasma and liver tissue malondialdehyde (MDA) and liver glutathione levels, as well as AST and ALT levels were studied. A histopathological examination was conducted. Results. Liver tissue MDA levels were found significantly lower in group 3, in comparison to group 2 (P<.05). Liver tissue MDA level in group 5 was significantly lower than that in group 4 (P<.001). Liver tissue glutathione levels were higher in group 5 and 3, relative to groups 4 and 2, respectively (P>.05 for each). Inflammation and focal necrosis decreased in group 3, in comparison to group 2 (P<.001 for each). Inflammation and focal necrosis in group 5 was lower than that in group 4 (P<.001). Actin expression decreased significantly in group 5, relative to group 4 (P<.05). Conclusion. Genistein has anti-inflammatory and antinecrotic effects on experimental liver damage caused by CCl4. Genistein reduces liver damage by preventing lipid peroxidation and strengthening antioxidant systems.

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Copyright © 2007 Nalan Kuzu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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