High Mobility Group Box 1 Protein Induction by <i>Mycobacterium Bovis</i> BCG

Hofner, Péter; Seprényi, György; Miczák, András; Buzás, Krisztina; Gyulai, Zsófia; Medzihradszky, Katalin F.; Rouhiainen, Ari; Rauvala, Heikki; Mándi, Yvette

doi:https://doi.org/10.1155/2007/53805

Mediators of Inflammation

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Research Article | Open Access

Volume 2007 | Article ID 053805 | https://doi.org/10.1155/2007/53805

High Mobility Group Box 1 Protein Induction by Mycobacterium Bovis BCG

Péter Hofner,¹György Seprényi,²András Miczák,¹Krisztina Buzás,³Zsófia Gyulai,¹Katalin F. Medzihradszky,⁴Ari Rouhiainen,⁵Heikki Rauvala,⁵and Yvette Mándi¹

Received01 Jun 2007

Accepted09 Oct 2007

Published15 Nov 2007

Abstract

High mobility group box 1 protein (HMGB1), a nuclear protein, is a critical cytokine that mediates the response to infection, injury, and inflammation. The aim of our study was to elaborate a reliable in vitro model to investigate whether Mycobacterium bovis BCG is able to induce HMGB1 secretion from the monocytic U-937 cells. Western blot technique was applied for the detection of HMGB1 from supernatants of cells, following induction with Mycobacterium bovis BCG. Densitometric analysis revealed higher concentrations of HMGB1 in cell supernatants stimulated with BCG than in the supernatants of the control, nonstimulated cells. Further quantitation of the secreted HMGB1 was performed by ELISA. The BCG strain resulted in a higher amount of secreted HMGB1 (450 ± 44 ng/mL) than that of LPS (84 ± 12 ng/mL) or Staphylococcus aureus (150 ± 14 ng/mL). BCG and Phorbol −12-myristate −13 acetate (PMA), added together, resulted in the highest HMGB1 secretion (645 ± 125 ng/mL). The translocation of the HMGB1 towards the cytoplasm following infection of cells with BCG was demonstrated by immunofluorescence examinations. Conclusion: Our pilot experiments draw attention to the HMGB1 inducing ability of Mycobacterium bovis. Assesment of the pathophysiological role of this late cytokine in mycobacterial infections demands further in vitro and in vivo examinations.

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Copyright © 2007 Péter Hofner et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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