Antioxidant Mechanism is Involved in the Gastroprotective Effects of Ozonized Sunflower Oil in Ethanol-Induced Ulcers in  Rats

Zamora Rodríguez, Zullyt B.; González Álvarez, Ricardo; Guanche, Dailén; Merino, Nelson; Hernández Rosales, Frank; Menéndez Cepero, Silvia; Alonso González, Yaima; Schulz, Siegfried

doi:https://doi.org/10.1155/2007/65873

Mediators of Inflammation

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Research Article | Open Access

Volume 2007 | Article ID 065873 | https://doi.org/10.1155/2007/65873

Antioxidant Mechanism is Involved in the Gastroprotective Effects of Ozonized Sunflower Oil in Ethanol-Induced Ulcers in Rats

Zullyt B. Zamora Rodríguez,¹Ricardo González Álvarez,¹Dailén Guanche,¹Nelson Merino,²Frank Hernández Rosales,¹Silvia Menéndez Cepero,¹Yaima Alonso González,¹and Siegfried Schulz³

Received31 Aug 2006

Revised04 Dec 2006

Accepted05 Dec 2006

Published17 Jan 2007

Abstract

This research was performed in order to determine the potential protective effects of ozonized sunflower oil (OSO) in the injury of rat gastric mucosa induced by absolute ethanol and as well as to elucidate the role of reactive oxygen species (ROS), lipid peroxidation, and some important constituents of antioxidant defense such as superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) in these effects. OSO was administered to rats intragastrically by a cannula and it was applied during four days to animals. The doses of OSO administered daily to each group of rats were 4, 12, and 24 mg/kg, respectively, and one hour after the last treatment, absolute ethanol (1 mL/200 mg body weight) was administered. Our results showed that gastric ulcer index was significantly reduced in rats pretreated with OSO as compared with ethanol-treated controls. However, in rats pretreated with OSO, no significant reduction of TBARS content in gastric mucosa was found as compared to those rats treated with ethanol alone. In contrast, SOD and GSH-Px activities were significantly increased in gastric mucosa of OSO-pretreated rats with respect to those treated with ethanol alone. In summary, our results demonstrate that OSO pretreatment exerts protective effects in ethanol-induced gastric ulcers in rats. Furthermore, these results provide evidence that these protective effects of OSO are mediated at least partially by stimulation of some important antioxidant enzymes such as SOD and GSH-Px, which are scavengers of ROS and therefore prevent gastric injury induced by them.

References

L. A. Sechi, I. Lezcano, N. Nuñez et al., “Antibacterial activity of ozonized sunflower oil (Oleozon),” Journal of Applied Microbiology, vol. 90, no. 2, pp. 279–284, 2001.
View at: Publisher Site | Google Scholar
A. Remigio, Y. A. González, Z. Zamora, and J. Moleiro, “Genotoxic evaluation of Oleozon by micronucleus assays in bone marrow and peripheral blood in mice,” Revista CENIC, Ciencias Biológicas, vol. 28, pp. 200–202, 1998.
View at: Google Scholar
Z. R. Zamora, D. Torres, M. Bouza, D. Hernández, and F. Hernández, “Oral ${Oleozon}^{®}$ , is an effective treatment in experimental giardiasis,” Revista CENIC Ciencias Biológicas, vol. 37, pp. 45–48, 2006.
View at: Google Scholar
P. R. Kvietys, B. Twohig, J. Danzell, and R. D. Specian, “Ethanol-induced injury to the rat gastric mucosa. Role of neutrophils and xanthine oxidase-derived radicals,” Gastroenterology, vol. 98, no. 4, pp. 909–920, 1990.
View at: Google Scholar
A. S. Salim, “Removing oxygen-derived free radicals stimulates healing of ethanol-induced erosive gastritis in the rat,” Digestion, vol. 47, no. 1, pp. 24–28, 1990.
View at: Google Scholar
S. Szabo, G. Pihan, and D. Dupuy, “The biochemical pharmacology of sulfhydryl compounds in gastric mucosal injury and protection,” in New Pharmacology of Ulcer Disease, S. Szabo and G. Mozik, Eds., pp. 424–446, Elsevier, New York, NY, USA, 1987.
View at: Google Scholar
B. Halliwell, “Reactive oxygen species in living systems: source, biochemistry, and role in human disease,” The American Journal of Medicine, vol. 91, no. 3, supplement 3, pp. S14–S22, 1991.
View at: Publisher Site | Google Scholar
B. Halliwell, J. M. C. Gutteridge, and C. E. Cross, “Free radicals, antioxidants, and human disease: where are we now?” Journal of Laboratory and Clinical Medicine, vol. 119, no. 6, pp. 598–620, 1992.
View at: Google Scholar
J. L. Wallace, C. M. Kennan, and O. N. Granger, “Gastric ulceration induced by non-steroidal anti-inflammatory drugs is a neutrophil dependent process,” American Journal of Physiology: Gastrointestinal and Liver Physiology, vol. 259, no. 3, part 1, pp. G462–G467, 1990.
View at: Google Scholar
V. Motilva, M. J. Martin, M. I. Luque, and C. Alarcón de la Lastra, “Role of polymorphonuclear leukocytes and oxygen-derived free radicals in chronic gastric lesion induced by acetic acid in rat,” General Pharmacology, vol. 27, no. 3, pp. 545–550, 1996.
View at: Publisher Site | Google Scholar
E. D. Jacobson, “Circulatory mechanisms of gastric mucosal damage and protection,” Gastroenterology, vol. 102, no. 5, pp. 1788–1800, 1992.
View at: Google Scholar
J. L. Wallace and B. C. Chin, “Inflammatory mediators in gastrointestinal defense and injury,” Proceedings of the Society for Experimental Biology and Medicine, vol. 214, no. 3, pp. 192–203, 1997.
View at: Google Scholar
V. N. Kochkov and N. Leitinger, “Anti-inflammatory properties of lipid oxidation products,” Journal of Molecular Medicine, vol. 81, no. 10, pp. 613–626, 2003.
View at: Publisher Site | Google Scholar
N. Leitinger, “Oxidized phospholipids as modulators of inflammation in atherosclerosis,” Current Opinion in Lipidology, vol. 14, no. 5, pp. 421–430, 2003.
View at: Publisher Site | Google Scholar
A. Robert, “Cytoprotection by prostaglandins,” Gastroenterology, vol. 77, no. 4, part 1, pp. 761–767, 1979.
View at: Google Scholar
H. Ohkawa, N. Ohishi, and K. Yagi, “Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction,” Analytical Biochemistry, vol. 95, no. 2, pp. 351–358, 1979.
View at: Publisher Site | Google Scholar
M. Minami and H. Yoshikawa, “A simplified assay method of superoxide dismutase activity for clinical use,” Clinica Chimica Acta, vol. 92, no. 3, pp. 337–342, 1979.
View at: Publisher Site | Google Scholar
B. Faraji, H. K. Kang, and J. L. Valentine, “Methods compared for determining glutathione peroxidase activity in blood,” Clinical Chemistry, vol. 33, no. 4, pp. 539–543, 1987.
View at: Google Scholar
C. Rice Evans and A. T. Diplock, “Laboratory techniques in biochemistry and molecular biology,” in Techniques in Free Radical Research, R. H. Burtin and P. H. Knippenberg, Eds., pp. 199–201, Elsevier, Amsterdam, The Netherlands, 1991.
View at: Google Scholar
O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall, “Protein measurement with the folin phenol reagent,” Journal of Biological Chemistry, vol. 193, no. 1, pp. 265–275, 1951.
View at: Google Scholar
C. La Casa, I. Villegas, C. Alarcón de la Lastra, V. Motilva, and M. J. Martín Calero, “Evidence for protective and antioxidant properties of rutin, a natural flavone, against ethanol induced gastric lesions,” Journal of Ethnopharmacology, vol. 71, no. 1-2, pp. 45–53, 2000.
View at: Publisher Site | Google Scholar
J. K. S. Ko, C. H. Cho, and C. W. Ogle, “The vagus nerve and its non-cholinergic mechanism in the modulation of ethanol-induced gastric mucosal damage in rats,” Journal of Pharmacy and Pharmacology, vol. 46, no. 1, pp. 29–33, 1994.
View at: Google Scholar
G. B. Glavin and S. Szabo, “Experimental gastric mucosal injury: laboratory models reveal mechanisms of pathogenesis and new therapeutic strategies,” FASEB Journal, vol. 6, no. 3, pp. 825–831, 1992.
View at: Google Scholar
R. Nordmann, C. Ribiere, and H. Rouach, “Implication of free radical mechanisms in ethanol-induced cellular injury,” Free Radical Biology and Medicine, vol. 12, no. 3, pp. 219–240, 1992.
View at: Publisher Site | Google Scholar
T. Brzozowski, P. C. Konturek, S. J. Konturek et al., “The role of melatonin and L-tryptophan in prevention of acute gastric lesions induced by stress, ethanol, ischemia, and aspirin,” Journal of Pineal Research, vol. 23, no. 2, pp. 79–89, 1997.
View at: Google Scholar
D. Billici, H. Süleyman, Z. N. Banoglu et al., “Melatonin prevents ethanol-induced gastric mucosal damage possibly due to its antioxidant effect,” Digestive Diseases and Sciences, vol. 47, no. 4, pp. 856–861, 2002.
View at: Publisher Site | Google Scholar
M. Kanter, H. Demir, C. Karakaya, and H. Ozbek, “Gastroprotective activity of Nigella sativa L oil and its constituent, thymoquinone against acute alcohol-induced gastric mucosal injury in rats,” World Journal of Gastroenterology, vol. 11, no. 42, pp. 6662–6666, 2005.
View at: Google Scholar

Copyright

Copyright © 2007 Zullyt B. Zamora Rodríguez et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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