Lipopolysaccharide Binding Protein and sCD14 are Not Produced as Acute Phase Proteins in Cardiac Surgery

Kudlova, Manuela; Kunes, Pavel; Kolackova, Martina; Lonsky, Vladimir; Mandak, Jiri; Andrys, Ctirad; Jankovicova, Karolina; Krejsek, Jan

doi:https://doi.org/10.1155/2007/72356

Mediators of Inflammation

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Clinical Study | Open Access

Volume 2007 | Article ID 072356 | https://doi.org/10.1155/2007/72356

Lipopolysaccharide Binding Protein and sCD14 are Not Produced as Acute Phase Proteins in Cardiac Surgery

Manuela Kudlova,¹Pavel Kunes,^1,2Martina Kolackova,¹Vladimir Lonsky,²Jiri Mandak,²Ctirad Andrys,¹Karolina Jankovicova,¹and Jan Krejsek¹

Received10 Jul 2007

Accepted04 Sept 2007

Published30 Oct 2007

Abstract

Objectives. The changes in the serum levels of lipopolysaccharide binding protein (LBP) and sCD14 during cardiac surgery were followed in this study. Design. Thirty-four patients, 17 in each group, were randomly assigned to coronary artery bypass grafting surgery performed either with (“on-pump”) or without (“off-pump”) cardiopulmonary bypass. LBP and sCD14 were evaluated by ELISA. Results. The serum levels of LBP were gradually increased from the 1st postoperative day and reached their maximum on the 3rd postoperative day in both “on-pump” and “off-pump” patients (30.33±9.96μg/mL; 37.99±16.58μg/mL), respectively. There were no significant differences between “on-pump” and “off-pump” patients regarding LBP. The significantly increased levels of sCD14 from the 1st up to the 7th postoperative day in both “on-pump” and “off-pump” patients were found with no significant differences between these groups. No correlations between LBP and sCD14 and IL-6, CRP and long pentraxin PTX3 levels were found. Conclusions. The levels of LBP and sCD14 are elevated in cardiac surgical patients being similar in both groups. These molecules are not produced as acute phase proteins in these patients.

References

L. A. Brasil, W. J. Gomes, R. Salomão, and E. Buffolo, “Inflammatory response after myocardial revascularization with or without cardiopulmonary bypass,” The Annals of Thoracic Surgery, vol. 66, no. 1, pp. 56–59, 1998.
View at: Publisher Site | Google Scholar
J. H. Levy and K. A. Tanaka, “Inflammatory response to cardiopulmonary bypass,” The Annals of Thoracic Surgery, vol. 75, no. 2, pp. S715–S720, 2003.
View at: Publisher Site | Google Scholar
M. S. Chew, I. Brandslund, V. Brix-Christensen et al., “Tissue injury and the inflammatory response to pediatric cardiac surgery with cardiopulmonary bypass: a descriptive study,” Anesthesiology, vol. 94, no. 5, pp. 745–753, 2001.
View at: Publisher Site | Google Scholar
K. M. Ubenauf, M. Krueger, P. Henneke, and R. Berner, “Lipopolysaccharide binding protein is a potential marker for invasive bacterial infections in children,” Pediatric Infectious Disease Journal, vol. 26, no. 2, pp. 159–162, 2007.
View at: Publisher Site | Google Scholar
D. C. Hargreaves and R. Medzhitov, “Innate sensors of microbial infection,” Journal of Clinical Immunology, vol. 25, no. 6, pp. 503–510, 2005.
View at: Publisher Site | Google Scholar
P. Kunes, V. Lonsky, J. Mandak et al., “The long pentraxin 3 in cardiac surgery: distinct responses in “on-pump” and “off-pump” patients,” Scandinavian Cardiovascular Journal, vol. 41, no. 3, pp. 171–179, 2007.
View at: Publisher Site | Google Scholar
J. Weiss, “Bactericidal/permeability-increasing protein (BPI) and lipopolysaccharide-binding protein (LBP): structure, function and regulation in host defence against Gram-negative bacteria,” Biochemical Society Transactions, vol. 31, no. 4, pp. 785–790, 2003.
View at: Publisher Site | Google Scholar
J. Zweigner, R. R. Schumann, and J. R. Weber, “The role of lipopolysaccharide-binding protein in modulating the innate immune response,” Microbes and Infection, vol. 8, no. 3, pp. 946–952, 2006.
View at: Publisher Site | Google Scholar
E. Fransen, J. Maessen, M. Dentener, N. Senden, and W. Buurman, “Impact of blood transfusions on inflammatory mediator release in patients undergoing cardiac surgery,” Chest, vol. 116, no. 5, pp. 1233–1239, 1999.
View at: Publisher Site | Google Scholar
M. A. Dentener, A. C. E. Vreugdenhil, P. H. M. Hoet et al., “Production of the acute-phase protein lipopolysaccharide-binding protein by respiratory type II epithelial cells: implications for local defense to bacterial endotoxins,” American Journal of Respiratory Cell and Molecular Biology, vol. 23, no. 2, pp. 146–153, 2000.
View at: Google Scholar
J. Zweigner, H.-J. Gramm, O. C. Singer, K. Wegscheider, and R. R. Schumann, “High concentrations of lipopolysaccharide-binding protein in serum of patients with severe sepsis or septic shock inhibit the lipopolysaccharide response in human monocytes,” Blood, vol. 98, no. 13, pp. 3800–3808, 2001.
View at: Publisher Site | Google Scholar
A. C. E. Vreugdenhil, C. H. Rousseau, T. Hartung, J. W. M. Greve, C. van 't Veer, and W. A. Buurman, “Lipopolysaccharide (LPS)-binding protein mediates LPS detoxification by chylomicrons,” The Journal of Immunology, vol. 170, no. 3, pp. 1399–1405, 2003.
View at: Google Scholar
M. Pavcnik-Arnol, S. Hojker, and M. Derganc, “Lipopolysaccharide-binding protein in critically ill neonates and children with suspected infection: comparison with procalcitonin, interleukin-6, and C-reactive protein,” Intensive Care Medicine, vol. 30, no. 7, pp. 1454–1460, 2004.
View at: Publisher Site | Google Scholar
S. Bas, B. R. Gauthier, U. Spenato, S. Stingelin, and C. Gabay, “CD14 is an acute-phase protein,” The Journal of Immunology, vol. 172, no. 7, pp. 4470–4479, 2004.
View at: Google Scholar
R. W. Finberg, F. Re, L. Popova, D. T. Golenbock, and E. A. Kurt-Jones, “Cell activation by Toll-like receptors: role of LBP and CD14,” Journal of Endotoxin Research, vol. 10, no. 6, pp. 413–418, 2004.
View at: Publisher Site | Google Scholar
G. Schmitz and E. Orsó, “CD14 signalling in lipid rafts: new ligands and co-receptors,” Current Opinion in Lipidology, vol. 13, no. 5, pp. 513–521, 2002.
View at: Publisher Site | Google Scholar
N. Hiki, D. Berger, Y. Mimura et al., “Release of endotoxin-binding proteins during major elective surgery: role of soluble CD14 in phagocytic activation,” World Journal of Surgery, vol. 24, no. 5, pp. 499–506, 2000.
View at: Publisher Site | Google Scholar
S. Takeshita, K. Nakatani, H. Tsujimoto, Y. Kawamura, H. Kawase, and I. Sekine, “Increased levels of circulating soluble CD14 in Kawasaki disease,” Clinical & Experimental Immunology, vol. 119, no. 2, pp. 376–381, 2000.
View at: Publisher Site | Google Scholar
C. Kruger, C. Schutt, U. Obertacke et al., “Serum CD14 levels in polytraumatized & severely burned patients,” Clinical & Experimental Immunology, vol. 85, no. 2, pp. 297–301, 1991.
View at: Google Scholar
M. A. Arias, J. E. Rey Nores, N. Vita et al., “Cutting edge: human B cell function is regulated by interaction with soluble CD14: opposite effects on IgG1 and IgE production,” The Journal of Immunology, vol. 164, no. 7, pp. 3480–3485, 2000.
View at: Google Scholar

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Copyright © 2007 Manuela Kudlova et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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