Protecting Effects of Dexamethasone on Thymus of Rats with Severe Acute Pancreatitis

Xiping, Zhang; Li, Chen; Miao, Lin; Hua, Tian

doi:https://doi.org/10.1155/2007/72361

Mediators of Inflammation

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Research Article | Open Access

Volume 2007 | Article ID 072361 | https://doi.org/10.1155/2007/72361

Protecting Effects of Dexamethasone on Thymus of Rats with Severe Acute Pancreatitis

Zhang Xiping,¹Chen Li,²Lin Miao,³and Tian Hua²

Received04 Sept 2007

Accepted05 Nov 2007

Published17 Dec 2007

Abstract

Purpose. To study the protecting effects of dexamethasone on thymus of rats with severe acute pancreatitis (SAP). Methods. The SAP rats were randomly assigned to the model group and dexamethasone-treated group, the other normal healthy rats were assigned to the sham operation group. The rat survival, thymus pathological changes, apoptotic index, as well as expression levels of NF-κB, P-selectin, Bax, Bcl-2, and Caspase-3 protein of all groups were observed, respectively, at 3 hours, 6 hours, and 12 hours. The contents of amylase and endotoxin in plasma as well as the contents of TNF-α, PLA2, and NO in serum were determined. Results. There was no marked difference between the model group and treated group in survival. The contents of different indexes in blood of treated group were lower than those of the model group to various degrees at different time points. The thymus pathological score was lower in treated group than in model group at 12 hours.The treated group in Caspase-3 protein expression of thymus significantly exceeded the model group at 12 hours. The apoptotic index was significantly higher in treated group than in model group. Conclusion. Dexamethasone has protecting effects on thymus of SAP rats.

References

T. Ueda, Y. Takeyama, T. Yasuda et al., “Significant elevation of serum interleukin-18 levels in patients with acute pancreatitis,” Journal of Gastroenterology, vol. 41, no. 2, pp. 158–165, 2006.
View at: Publisher Site | Google Scholar
S. Uehara, K. Gothoh, H. Handa, H. Tomita, and Y. Tomita, “Immune function in patients with acute pancreatitis,” Journal of Gastroenterology and Hepatology, vol. 18, no. 4, pp. 363–370, 2003.
View at: Publisher Site | Google Scholar
J.-X. Zhang, S.-C. Dang, J.-G. Qu, X.-Q. Wang, and G.-Z. Chen, “Changes of gastric and intestinal blood flow, serum phospholipase A2 and interleukin-1 $β$ in rats with acute necrotizing pancreatitis,” World Journal of Gastroenterology, vol. 11, no. 23, pp. 3578–3581, 2005.
View at: Google Scholar
Y. Takeyama, “Significance of apoptotic cell death in systemic complications with severe acute pancreatitis,” Journal of Gastroenterology, vol. 40, no. 1, pp. 1–10, 2005.
View at: Publisher Site | Google Scholar
X. Wang, B. Wang, K. Wu, M. Xu, and Z. Gong, “Growth hormone downregulated the excessive apoptosis of ileal intestinal epithelial cells in rats during the early course of acute necrotizing pancreatitis,” Pancreas, vol. 25, no. 2, pp. 205–209, 2002.
View at: Publisher Site | Google Scholar
S. Cheng, M. M. Song, Z. H. Li, and S. G. He, “The protective role of gadolinium chloride in lung injury associated with acute necrotizing pancreatitis,” Zhonghua Wai Ke Za Zhi, vol. 42, no. 15, pp. 936–939, 2004.
View at: Google Scholar
Y. Takeyama, J. Nishikawa, T. Ueda, and Y. Hori, “Thymic atrophy caused by thymocyte apoptosis in experimental severe acute pancreatitis,” Journal of Surgical Research, vol. 78, no. 2, pp. 97–102, 1998.
View at: Publisher Site | Google Scholar
X. Zhang, Q. Ye, X.-G. Jiang et al., “Preparation method of an ideal model of multiple organ injury of rat with severe acute pancreatitis,” World Journal of Gastroenterology, vol. 13, no. 34, pp. 4566–4573, 2007.
View at: Google Scholar
X. Zhang, L. Chen, Q.-F. Hu et al., “Effects of large dose of dexamethasone on inflammatory mediators and pancreatic cell apoptosis of rats with severe acute pancreatitis,” World Journal of Gastroenterology, vol. 13, no. 41, pp. 5506–5511, 2007.
View at: Google Scholar
X. Zhang, L. Zhang, Y. Wang et al., “Study of the protective effects of dexamethasone on multiple organ injury in rats with severe acute pancreatitis,” Journal of the Pancreas, vol. 8, no. 4, pp. 400–412, 2007.
View at: Google Scholar
O. Z. Saĭdalikhodzhaeva, N. M. Iuldashev, A. N. Daniiarov, and U. Z. Muratova, “Pancreatic enzyme activity in early phases of acute experimental pancreatitis in rats,” Ross Fiziol Zh Im I M Sechenova, vol. 88, no. 4, pp. 526–529, 2002.
View at: Google Scholar
S. H. Um, Y. D. Kwon, C. D. Kim et al., “The role of nitric oxide in experimental cerulein induced pancreatitis,” Journal of Korean Medical Science, vol. 18, no. 4, pp. 520–526, 2003.
View at: Google Scholar
A. Gulcubuk, K. Altunatmaz, K. Sonmez et al., “Effects of curcumin on tumour necrosis factor- $a$ and interleukin-6 in the late phase of experimental acute pancreatitis,” Journal of Veterinary Medicine A, vol. 53, no. 1, pp. 49–54, 2006.
View at: Publisher Site | Google Scholar
L. Kerekes, P. Antal-Szalmás, B. Dezso et al., “In vitro examination of the influence of lipase and amylase on dog's pancreas tissue incubated with endotoxins, phospholipase A2 or cytokines,” Magyar Sebeszet, vol. 58, no. 2, pp. 120–124, 2005.
View at: Google Scholar
C. Vasilescu, V. Herlea, K. Buttenschoen, and H. G. Beger, “Endotoxin translocation in two models of experimental acute pancreatitis,” Journal of Cellular and Molecular Medicine, vol. 7, no. 4, pp. 417–424, 2003.
View at: Publisher Site | Google Scholar
J. Aufenanger, M. Samman, M. Quintel, K. Fassbender, W. Zimmer, and T. Bertsch, “Pancreatic phospholipase A2 activity in acute pancreatitis: a prognostic marker for early identification of patients at risk,” Clinical Chemistry and Laboratory Medicine, vol. 40, no. 3, pp. 293–297, 2002.
View at: Publisher Site | Google Scholar
Y. Tomita, K. Kuwabara, S. Furue et al., “Effect of a selective inhibitor of secretory phospholipase A2, S-5920/LY315920Na, on experimental acute pancreatitis in rats,” Journal of Pharmacological Sciences, vol. 96, no. 2, pp. 144–154, 2004.
View at: Publisher Site | Google Scholar
E. Vaquero, I. Gukovsky, V. Zaninovic, A. S. Gukovskaya, and S. J. Pandol, “Localized pancreatic NF- $κ$ B activation and inflammatory response in taurocholate-induced pancreatitis,” American Journal of Physiology, vol. 280, no. 6, pp. G1197–G1208, 2001.
View at: Google Scholar
J. Long, N. Song, X.-P. Liu, K.-J. Guo, and R.-X. Guo, “Nuclear factor- $κ$ B activation on the reactive oxygen species in acute necrotizing pancreatitic rats,” World Journal of Gastroenterology, vol. 11, no. 27, pp. 4277–4280, 2005.
View at: Google Scholar
A. H. Lundberg, D. N. Granger, J. Russell et al., “Quantitative measurement of P- and E-selectin adhesion molecules in acute pancreatitis: correlation with distant organ injury,” Annals of Surgery, vol. 231, no. 2, pp. 213–222, 2000.
View at: Publisher Site | Google Scholar
H. Kameda, I. Morita, M. Handa et al., “Re-expression of functional P-selectin molecules on the endothelial cell surface by repeated stimulation with thrombin,” British Journal of Haematology, vol. 97, no. 2, pp. 348–355, 1997.
View at: Publisher Site | Google Scholar
S. Ushiyama, T. M. Laue, K. L. Moore, H. P. Erickson, and R. P. McEver, “Structural and functional characterization of monomeric soluble P-selectin and comparison with membrane P-selectin,” Journal of Biological Chemistry, vol. 268, no. 20, pp. 15229–15237, 1993.
View at: Google Scholar
M. Hénaff, S. Antoine, J. J. Mercadier, A. Coulombe, and S. N. Hatem, “The voltage-independent B-type ${Ca}^{2 +}$ channel modulates apoptosis of cardiac myocytes,” The FASEB Journal, vol. 16, no. 1, pp. 99–101, 2002.
View at: Google Scholar
G. Nuñez, M. A. Benedict, Y. Hu, and N. Inohara, “Caspases: the proteases of the apoptotic pathway,” Oncogene, vol. 17, no. 25, pp. 3237–3245, 1998.
View at: Google Scholar
D. Malka, S. Vasseur, H. Bödeker et al., “Tumor necrosis factor $a$ triggers antiapoptotic mechanisms in rat pancreatic cells through pancreatitis-associated protein I activation,” Gastroenterology, vol. 119, no. 3, pp. 816–828, 2000.
View at: Publisher Site | Google Scholar
Y. Nagashio, H. Ueno, M. Imamura et al., “Inhibition of transforming growth factor $ß$ decreases pancreatic fibrosis and protects the pancreas against chronic injury in mice,” Laboratory Investigation, vol. 84, no. 12, pp. 1610–1618, 2004.
View at: Publisher Site | Google Scholar
L. Leindler, É. Morschl, F. László et al., “Importance of cytokines, nitric oxide, and apoptosis in the pathological process of necrotizing pancreatitis in rats,” Pancreas, vol. 29, no. 2, pp. 157–161, 2004.
View at: Publisher Site | Google Scholar
Y. Peng, S. F. Gallagher, K. Haines, K. Baksh, and M. M. Murr, “Nuclear factor- $κ$ B mediates Kupffer cell apoptosis through transcriptional activation of Fas/FasL,” Journal of Surgical Research, vol. 130, no. 1, pp. 58–65, 2006.
View at: Publisher Site | Google Scholar
M. Chvanov, O. H. Petersen, and A. Tepikin, “Free radicals and the pancreatic acinar cells: role in physiology and pathology,” Philosophical Transactions of the Royal Society B, vol. 360, no. 1464, pp. 2273–2284, 2005.
View at: Publisher Site | Google Scholar
H. H. Pei, R.-L. Liu, C.-Q. Jiang, L. L. Ma, and X.-Y. Fang, “Influence of emodin on pancreatitic acinar cell apoptosis in rats with acute pancreatitis,” Journal of Bengbu Medical College, vol. 30, no. 2, pp. 112–113, 2005 (Chinese).
View at: Google Scholar
H. H. Pei, W. Dai, and J. Zhou, “Effects of somatostatin on apoptosis of pancreatitic acinar cell apoptosis in acute necrotizing pancreatitis in rats,” Guangdong Medical Journal, vol. 25, no. 2, pp. 138–140, 2004 (Chinese).
View at: Google Scholar
K.-B. Hahm, J.-H. Kim, B.-M. You et al., “Induction of apoptosis with an extract of Artemisia asiatica attenuates the severity of cerulein-induced pancreatitis in rats,” Pancreas, vol. 17, no. 2, pp. 153–157, 1998.
View at: Publisher Site | Google Scholar
V. D. Samuilov, A. V. Oleskin, and E. M. Lagunova, “Programmed cell death,” Biochemistry, vol. 65, no. 8, pp. 873–887, 2000.
View at: Google Scholar
Y. Sugiyama, S. Kato, M. Abe, S. Mitsufuji, and K. Takeuchi, “Different effects of dexamethasone and the nitric oxide synthase inhibitor L-NAME on caerulein-induced rat acute pancreatitis, depending on the severity,” Inflammopharmacology, vol. 13, no. 1–3, pp. 291–301, 2005.
View at: Publisher Site | Google Scholar
Z.-F. Wang, C.-E. Pan, Y. Lu, S.-G. Liu, G.-J. Zhang, and X. Zhang, “The role of inflammatory mediators in severe acute pancreatitis and regulation of glucocorticoids,” Hepatobiliary and Pancreatic Diseases International, vol. 2, no. 3, pp. 458–462, 2003.
View at: Google Scholar
A. Paszt, T. Takács, Z. Rakonczay et al., “The role of the glucocorticoid-dependent mechanism in the progression of sodium taurocholate-induced acute pancreatitis in the rat,” Pancreas, vol. 29, no. 1, pp. 75–82, 2004.
View at: Publisher Site | Google Scholar
R. Dong, Z. F. Wang, Y. Lv, and Q. J. Ma, “Treatment of severe acute pancreatitis with large dosage of dexamethsone in the earlier time,” Journal of Hepatobiliary Surgery, vol. 13, no. 1, pp. 58–60, 2005 (Chinese).
View at: Google Scholar
R. Dong, Z. F. Wang, Y. Lv, and C. E. Pan, “Treatment of severe acute pancreatitis with large dosage of dexamethsone in rats,” Chinese Journal of General Surgery, vol. 10, no. 4, pp. 309–311, 2001 (Chinese).
View at: Google Scholar
M. Iwamori and Y. Iwamori, “Changes in the glycolipid composition and characteristic activation of GM3 synthase in the thymus of mouse after administration of dexamethasone,” Glycoconjugate Journal, vol. 22, no. 3, pp. 119–126, 2005.
View at: Publisher Site | Google Scholar
S. Bruscoli, R. Di Virgilio, V. Donato et al., “Genomic and non-genomic effects of different glucocorticoids on mouse thymocyte apoptosis,” European Journal of Pharmacology, vol. 529, no. 1–3, pp. 63–70, 2006.
View at: Publisher Site | Google Scholar
D. Wang, N. Müller, K. G. McPherson, and H. M. Reichardt, “Glucocorticoids engage different signal transduction pathways to induce apoptosis in thymocytes and mature T cells,” Journal of Immunology, vol. 176, no. 3, pp. 1695–1702, 2006.
View at: Google Scholar
T. Hajtò, T. Berki, L. Pàlinkàs, F. Boldizsàr, and P. Németh, “Effects of mistletoe extract on murine thymocytes in vivo and on glucocorticoid-induced cell count reduction,” Forschende Komplementärmedizin, vol. 13, no. 1, pp. 22–27, 2006.
View at: Publisher Site | Google Scholar
Y. Sun, J. Y. Lu, X. H. Wang, Y. Lang, and L. Li, “Pathologic changes of thymic dendritic cells in mouse MODS model induced by zymosan,” International Journal of Clinical and Experimental Pathology, vol. 21, no. 1, pp. 95–99, 2005 (Chinese).
View at: Google Scholar
H. J. Aho, S. M. Koskensalo, and T. J. Nevalainen, “Experimental pancreatitis in the rats: sodium taurocholate-induced acute hemorrhagic pancreatitis,” Scandinavian Journal of Gastroenterology, vol. 15, no. 4, pp. 411–416, 1980.
View at: Google Scholar
H. Tian, X. Zhang, L. Chen, and G. H. Feng, “Technique improvement on traditional animal model with severe acute pancreatitis in rats,” Chinese Journal of Emergency Medicine, vol. 14, no. 3, pp. 211–214, 2005 (Chinese).
View at: Google Scholar
R. L. Parker, D. G. Huntsman, D. W. Lesack et al., “Assessment of interlaboratory variation in the immunohistochemical determination of estrogen receptor status using a breast cancer tissue microarray,” American Journal of Clinical Pathology, vol. 117, no. 5, pp. 723–728, 2002.
View at: Publisher Site | Google Scholar
T. Zellweger, C. Ninck, M. Mirlacher et al., “Tissue microarray analysis reveals prognostic significance of syndecan-1 expression in prostate cancer,” The Prostate, vol. 55, no. 1, pp. 20–29, 2003.
View at: Publisher Site | Google Scholar
C. Laprise, R. Sladek, A. Ponton, M. C. Bernier, T. J. Hudson, and M. Laviolette, “Functional classes of bronchial mucosa genes that are differentially expressed in asthma,” Journal of Histochemistry and Cytochemistry, vol. 52, no. 1, pp. 501–507, 2004.
View at: Google Scholar

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Copyright © 2007 Zhang Xiping et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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