Inhibitory Effect of Inflexinol on Nitric Oxide Generation and iNOS Expression via Inhibition of NF-<mml:math id="E1" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:mi>κ</mml:mi><mml:mtext>B</mml:mtext></mml:mrow></mml:math> Activation

Lee, Jae Woong; Lee, Moon Soon; Kim, Tae Hun; Lee, Hwa Jeong; Hong, Seong Su; Noh, Young Hee; Hwang, Bang Yeon; Ro, Jai Seup; Hong, Jin Tae

doi:https://doi.org/10.1155/2007/93148

Mediators of Inflammation

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Research Article | Open Access

Volume 2007 | Article ID 093148 | https://doi.org/10.1155/2007/93148

Inhibitory Effect of Inflexinol on Nitric Oxide Generation and iNOS Expression via Inhibition of NF-κB Activation

Jae Woong Lee,¹Moon Soon Lee,²Tae Hun Kim,¹Hwa Jeong Lee,¹Seong Su Hong,¹Young Hee Noh,³Bang Yeon Hwang,¹Jai Seup Ro,¹and Jin Tae Hong¹

Received06 Oct 2006

Accepted24 Jan 2007

Published28 Mar 2007

Abstract

Inflexinol, an ent-kaurane diterpenoid, was isolated from the leaves of Isodon excisus. Many diterpenoids isolated from the genus Isodon (Labiatae) have antitumor and antiinflammatory activities. We investigated the antiinflammatory effect of inflexinol in RAW 264.7 cells and astrocytes. As a result, we found that inflexinol (1, 5, 10 μM) suppressed the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) as well as the production of nitric oxide (NO) in LPS-stimulated RAW 264.7 cells and astrocytes. Consistent with the inhibitory effect on iNOS and COX-2 expression, inflexinol also inhibited transcriptional and DNA binding activity of NF-κB via inhibition of IκB degradation as well as p50 and p65 translocation into nucleus. These results suggest that inflexinol inhibits iNOS and COX-2 expression through inhibition of NF-κB activation, thereby inhibits generation of inflammatory mediators in RAW 264.7 cells and astrocytes, and may be useful for treatment of inflammatory diseases.

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Copyright © 2007 Jae Woong Lee et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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