Increased Responsiveness to Toll-Like Receptor 4 Stimulation in Peripheral Blood Mononuclear Cells from Patients with Recent Onset Rheumatoid Arthritis
Table 4
Relative
quantification of the TLR2 and TLR4 mRNA expression in PBMCs from patients with rheumatoid
arthritis (RA), osteoarthrosis (OA), and healthy control (HC).
(a) Target gene
expression is normalized to housekeeping gene expression and presented as n-fold of the expression in HC group ().
denotes significant difference in expression comparing with HC ().
Nonstimulated
Cytokine mix
LPS
TLR2
HC
1
1
1
RA
2.045
2.915
0.036
OA
1.218
1.66
0.074
TLR4
HC
1
1
1
RA
0.008
0.225
0.0009
OA
0.004
0.044
0.00002
(b) Target gene
expression after stimulation with
cytokine mix or LPS is normalized to housekeeping
gene expression and presented as n-fold
of the expression in non stimulated cells from patients from every group ().
Significant differences in expression compared to nonstimulated cells are
marked with .
