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Mediators of Inflammation
Volume 2008, Article ID 168652, 8 pages
http://dx.doi.org/10.1155/2008/168652
Research Article

Lipoperoxidation and Protein Oxidative Damage Exhibit Different Kinetics During Septic Shock

1Departamento de Medicina Intensiva, Facultad de Medicina, Pontificia Universidad Católica de Chile, Alameda, 340 Santiago, Chile
2Departamento de Biología Celular y Molecular, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Alameda, 340 Santiago, Chile
3Facultad de Matematicas, Pontificia Universidad Católica de Chile, Alameda, 340 Santiago, Chile

Received 1 January 2008; Revised 28 March 2008; Accepted 5 May 2008

Academic Editor: V. L. Petricevich

Copyright © 2008 Max Andresen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Septic shock (SS)-related multiorgan dysfunction has been associated with oxidative damage, but little is known about the temporal damage profile and its relationship to severity. The present work investigated prospectively 21 SS patients. Blood samples were obtained at diagnosis, 24, 72 hours, day 7, and at 3 months. At admission, thiobarbituric acid reactive substances (TBARSs), plasma protein carbonyls, plasma protein methionine sulfoxide (MS), ferric/reducing antioxidant power (FRAP), total red blood cell glutathione (RBCG), uric acid (UA), and bilirrubin levels were increased ( ). Total radical—trapping antioxidant potential (TRAP) and vitamin-E were similar to controls, and vitamin-C was decreased ( ). During evolution, TBARS and RBCG increased ( ), vitamin-E levels remained stable, whereas plasma protein carbonyls and MS, TRAP, vitamin-C, reduced glutathione, and UA levels decreased ( ). After 3 months, plasma protein carbonyls and MS persisted elevated. More severe patients exhibited higher TBARS, TRAP, FRAP, vitamin-C, UA, and bilirrubin levels. Our results suggest early and persistent oxidative stress during septic shock and a correlation between increasing levels of lipoperoxidation and sepsis severity.