Suppressive Effect of Hydroquinone, a Benzene Metabolite, on In Vitro Inflammatory Responses Mediated by Macrophages, Monocytes, and Lymphocytes
Figure 3
Effects
of hydroquinone on the production of NO in LPS-treated RAW 264.7 cells or
peritoneal macrophages. (a) and (b) RAW264.7 cells or peritoneal macrophages () were pretreated with various
concentrations of HQ
(hydroquinone) in the presence or absence of LPS (2 g/mL), PGN
(10 g/mL),
or
zymosan (400 g/mL) for
24 hours. The level of NO was determined by Griess reagent as described in
Section 2. (c) The mRNA levels of proinflammatory
cytokines from RAW264.7 cells were determined by semiquantitative RT-PCR. The results show one representative
experiment of three. and represent
significant difference as compared to control.
