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Mediators of Inflammation
Volume 2008, Article ID 512196, 7 pages
http://dx.doi.org/10.1155/2008/512196
Research Article

Staphylococcal Toxic Shock Syndrome Toxin-1 Induces the Translocation and Secretion of High Mobility Group-1 Protein from Both Activated T Cells and Monocytes

1Vancouver Hospital & Health Sciences, Diamond Health Care Centre, Department of Medicine, Vancouver, BC, Canada V5Z 1M9
2Division of Infectious Diseases, Department of Medicine, University of British Columbia, Vancouver, BC, Canada V5Z 3J5

Received 6 August 2008; Accepted 26 September 2008

Academic Editor: Tania Fröde

Copyright © 2008 Shirin Kalyan and Anthony W. Chow. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

High mobility group box-1 (HMGB-1) is a DNA-binding protein secreted by activated monocytes and has been identified as a key late mediator of endotoxic shock. We investigated the regulation of HMGB-1 in human peripheral blood mononuclear cells (PBMCs) following stimulation with the staphylococcal superantigen, toxic shock syndrome toxin-1 (TSST-1), and found that TSST-1, like LPS, induced the secretion of HMGB-1 from human PBMC. However, unlike monocyte-driven sepsis caused by endotoxin, translocation and secretion of HMGB-1 mediated by TSST-1 was dependent on the presence of both activated T cells and monocytes. Furthermore, we show that nuclear HMGB-1 is released from TSST-1 stimulated T cells. This finding presents a basis for investigating the potential of targeting HMGB-1 for the treatment of toxic shock syndrome, and provides further insight on the role of HMGB-1 in the cross-talk between activated monocytes and T cells.