Staphylococcal Toxic Shock Syndrome Toxin-1 Induces the Translocation and Secretion of High Mobility Group-1 Protein from Both Activated T Cells and Monocytes
Figure 1
Secretion of membrane-associated HMGB-1 in the extracellular milieu following TSST-1
treatment. Extracellular expression of HMGB-1 on adherent PBMC in
complete growth medium (a) or after TSST-1 treatment. (b) Anti-HMGB-1: green
fluorescence (Alexa488); nucleus: blue (Hoechst 3342 nuclear dye); anti-CD3:
red (Alexa594). Close-up views from
representative fields are depicted in the left panels. (c) Flow cytometry
analysis of surface-expressed HMGB-1 in PBMC 24 hours following TSST-1
stimulation. Left panel, resting or PBMC treated; right panel, 24 hours after
TSST-1 stimulation. Surface HMGB-1 expression significantly decreased after
TSST-1 stimulation (12.0% versus 4.3% in the donor shown; % prior to
stimulation versus % post-TSST-1 treatment from 4 different donors; , paired Student's t-test).
