Staphylococcal Toxic Shock Syndrome Toxin-1 Induces the Translocation and Secretion of High Mobility Group-1 Protein from Both Activated T Cells and Monocytes
Figure 2
Nuclear
translocation of HMGB-1 in human PBMC following TSST-1 stimulation. Human PBMCs were treated with either 1 nM TSST-1 or RPMI medium for 10 hours,
and the intracellular expression of HMGB-1 was detected by confocal fluorescent
microscopy. Close-up views from representative fields are depicted in the right
panels. (a) Resting (RPMI) PBMC, intracellular HMGB-1 (green) was seen to be
localized primarily in the nucleus (blue). (b) At 10 hours following
stimulation with 1 nM TSST-1, HMGB-1 was seen to translocate into the
extracellular space. (c) Fluorescent microscopy image of resting T cells, and (d)
TSST-1 stimulated T cells in the process of actively translocating intracellular HMGB-1 10 hours following toxin
treatment. T cells were detected by staining with antihuman CD3
antibodies conjugated to Alexa594 (red). (e) Detection of HMGB-1 by immunoblot
in the culture supernatant of human PBMC 24 hours after treatment with 1 nM
TSST-1 or RPMI medium.
